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《Journal of Anhui Agricultural Sciences》 2008-27
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Genetic Analysis of Small Grain Mutant Induced by T-DNA Insertion in Rice

ZHANG Xiang-qian et al(College of Agriculture,South China Agricultural University,Guangzhou,Guangdong 510642)  
[Objective] The aim of this study was to understand the genetic characteristics of a grain shape mutant and its possible role in genetic improvement of grain yield in rice.[Method] On the basis of the collection of T-DNA tag lines,the progeny of homozygous plants carrying T-DNA insertion were screened for mutants with mutated phenotypes.The genetic analysis of the mutant and test for the linkage between the mutated phenotype and the T-DNA insertion were carried out to determine its genetic characteristics.[Result] In the present study,a grain shape mutant induced by T-DNA insertion in rice was identified,which showed small grain in mutants.Genetic analysis of the mutant showed that the two types of phenotype,normal and small grain in the segregating populations derived from the T-DNA heterozygotes,fiting the ratio of 3∶1.Test for Basta resistance showed that all of the mutants were resistant while the normal plants segregated for resistant and susceptible by the ratio of 2∶1.The results indicated that the mutant phenotype co-segregated with Bar gene.The small grain mutant caused by T-DNA insertion was confirmed by PCR amplification aiming at T-DNA.[Conclusion] The grain shape mutant is useful for isolation of the tagged gene and genetic improvement in rice.
【Fund】: 国家自然科学基金(3067128)资助
【CateGory Index】: S511
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