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《Journal of Anhui Agricultural Sciences》 2008-28
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Preliminary Study on the Insecticidal Active Substances in the Roots of Gentiana macrophylla against Plutella xylostella

LIU Shi-wei et al (Department of Chemical Engineering,Ningxia Teachers University,Guyuan,Ningxia 756000)  
[Objective] The research aimed to study the insecticidal active substances in the roots of Gentiana macrophylla against Plutella xylostella.[Method] The active substances in the roots of G.macrophylla were extracted by ethanol ultrasonic method.The contact activity of the extracts against P.xylostella was tested by using insect-dipping method.The extracts were leached with petroleum ether,chloroform,ethyl acetate and n-butanol by using liquid-liquid allocation extraction method in order.The tracking test was made on the activity of each extract.[Result] LC50 of the extract from the roots of P.xylostella to the 3rd-instar larva was 0.795 8 g/ml.The activity of petroleum ether extract was strongest after separating the extracts,followed by chloroform extract and ethyl acetate extract.But the effects of n-butanol extract and remainder in water layer were not obvious.[Conclusion] The roots of G.macrophylla had stronger contact activity against P.xylostella and the insecticidal active substances were liposoluble compounds.
【Fund】: 宁夏自然科学基金资助项目(NZ08171);; 宁夏高等学校科学研究基金资助项目(2006237);; 宁夏师范学院基金资助项目(Z07002);; 国家自然科学基金资助项目(20565003)
【CateGory Index】: S482.3
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