Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui Agricultural Sciences》 2008-29
Add to Favorite Get Latest Update

Establishment of DNA Extraction and ISSR-PCR Reacting System of Tutcheria kweichowensis

ZHAO Yang et al (College of Forestry,Guizhou University,Guiyang,Guizhou 550225)  
[Objective] The research aimed to lay the foundation for the genetic diversity analysis of Tutcheria kweichowensis by ISSR technology.[Method] The materials of the fresh leaves,dry leaves and seeds of Tutcheria kweichowensis and the improved methods of SDS and CTAB were used for the DNA extraction experiment,and the concentration and purity of the extracted DNA were determined.Through single-factor gradient test,the optimized ISSR-PCR reaction system was screened out.[Result] When extracting the leaf DNA of Tutcheria kweichowensis with the methods of CTAB and SDS,there were small differences in quality and density between the fresh leaves and dry leaves.There were higher density and more protein residues by CTAB than those by SDS.The quantity of DNA with SDS was a little less than that of CTAB but with the highest quality.Both the quality and density of DNA extracted from seeds were worse,but still qualified for ISSR molecular marking experiment.The appropriate ISSR-PCR reacting conditions were as follows: 20 ul reacting system,10×Buffer,1 U Taq DNA enzyme,0.2 μmol/L primer,0.25 mmol/L L dNTP and 40 ng template DNA.[Conclusion] This ISSR reaction system was suitable for the genetic diversity analysis of Tutcheria kweichowensis.
【Fund】: 贵州大学引进人才基金项目(X060055)
【CateGory Index】: Q943.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 3 Hits
1 MAO Yun-ling et al (Yunnan Academy of Forestry,Yunnan Key Laboratory for the Protection and Propagation of Rare and Endangered Forest Plants of State Forestry Bureau,Key Laboratory for the Cultivation and Exploitation of Forest Plants in Yunnan Province,Kunming,Yunnan 650204);Establishment of ISSR-PCR Reaction System for the Rare and Endangered Plant Manglietia grandis Hu et Cheng[J];Journal of Anhui Agricultural Sciences;2009-31
2 Cui Yong;Guangxi Vocational and Technical College;;Study on resources of Tutcheria Plants and their horticulture & gardening practice in China[J];Journal of Guangxi Agriculture;2015-02
3 CHEN Xiang,TIAN Xue-qin,WANG Zhi-yun,YIN Ai-hua,KE Huan(Foshan Institute of Forestry Science,Foshan 528222,Guangdong,China);Effects of different substrates on container-seedlings of six native species[J];Journal of Central South University of Forestry & Technology;2013-05
【Citations】
Chinese Journal Full-text Database 6 Hits
1 LIU Zun-chun et al(Henan Institute of Science and Technology,Xinxiang,Henan 453003);Comparison of Genomic DNA Extraction Methods of Jinhua Pear[J];Journal of Anhui Agricultural Sciences;2005-11
2 XUE Zhi-zhong et al(College of Biological Science and Engineering,Hebei University of Science and Technology,Shijiazhuang,Hebei 050018);DNA Extraction from Oil Sunflower Seeds and the Optimization of Its RAPD Reaction System[J];Journal of Anhui Agricultural Sciences;2008-06
3 Zou Tiancai, Jin ping (Guizhou Botanical Garden, Guiyang 550001);INVESTIGATION AND APPROACH ON RESOURCES AND UTILIZATION OF TUTCHERIA KWEICHOUENSIS CHANG ET Y.K.LI[J];Guizhou Science;1996-04
4 BIN Xiao-Yun~1,TANG Shao-Qing~1~~,ZHOU Jun-Ya~1,SONG Hong-Tao~2,LI Zun-Yi~3 (1.College of Life Sciences,Guangxi Normal University,Guilin541004,China;2.Fangcheng Golden Camellia Nature Reserve,Fangcheng,Guangxi538021,China;3.Fenghuangshan Forestry Farm,Fusui,Guangxi532114,China);ISSR Analysis on Genetic Diversity of Camellia nitidissima Chi (Theaceae) in China[J];Journal of Wuhan Botanical Research;2005-01
5 CHEN Jin1,Dilbar TOHTY1,GUO Weidong2(1.School of Life Science and Chemistry,Xinjiang Normal University,Urumqi Xinjiang 830054;2.College of Chemistry and life Science,Zhejiang Normal University,Jinhua Zhejiang 321000);A Comparative Study of Five Methods of the Genomic DNA Isolation from Sarcandra Glabra(Thunb.) Nakai Leaves[J];Journal of Xinjiang Normal University(Natural Sciences Edition);2008-01
6 ZHAO Yang~1,CHEN Xiao-yang~1,LI Tong-sen~2,HU Dong-nan~1(1.The Laboratory of Genetics and Breeding of Trees and Flowers,Beijing Forestry University,Beijing 100083,China;2.Faculty of Landscape Architecture,Southwest Forestry College,Kunming Yunnan 650224,China);Establishment and Optimization of ISSR Reaction System for Lespedeza spp.[J];Journal of Southwest Forestry College;2006-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Ji-zhen,LIU Ya-qiong,YANG Bing,WANG Xian*(College of Forestry and Horticulture,Henan Agricultural University,Zhenzhou 450002,China);ISSR Molecular Marker and Its Application in Garden Plant Genetics and Breeding[J];Shandong Agricultural Sciences;2008-08
2 Luo Haiyan et al.(1 Tropical Crops Genetic Resources Institutes,Chinese Academy of Tropical Agriculture Sciences,Hainan 571737,China;2 Chinese Academy of Tropical Agriculture Sciences,Hainan 571737,China);ISSR Molecular Marker and its Using[J];Anhui Agricultural Science Bulletin;2008-19
3 Lv Yanzhen et al.(School of Life Science,Huaibei Normal University,Huaibei 235000,China;Anhui Key Laboratory of Plant Resources and Biology,Huaibei 235000,China);Optimization of ISSR Reaction system in Carya cathayensis[J];Anhui Agricultural Science Bulletin;2011-07
4 LIU Feng et al(Zhejiang Normal University,Chemistry and Life Science,Jinhua,Zhejiang 321004);Research on the Orthogonal Optimization of ISSR-PCR Reaction System of Madagascar Periwinkle[J];Journal of Anhui Agricultural Sciences;2010-05
5 WANG Zhen et al(College of Life and Environmental Sciences,Minzu University of China,Beijing 100081);Optimization for ISSR-PCR Reaction System on Artemisia frigida Willd. Using Orthogonal Design[J];Journal of Anhui Agricultural Sciences;2010-15
6 WANG Lan et al(College of Life Sciences,Sichuan University,Chengdu,Sichuan 610064);Establishment and Optimization of ISSR-PCR Amplification System for Ligusticum chuanxiong Hort[J];Journal of Anhui Agricultural Sciences;2011-23
7 XIE Yun et al(Research Institute of Subtropical Forestry,Chinese Academy of Forestry,Fuyang,Zhejiang 311400);DNA Extraction and ISSR Primer Screening of Camellia chekiangoleosa[J];Journal of Anhui Agricultural Sciences;2011-25
8 Li Rong,Niu Jian-xin(Agricultural College of Shihezi University,Shihezi,Xinjiang 832003,China);AFLP molecular marker technique and its application in the studies of fruit trees[J];Northern Fruits;2006-05
9 CHU Yun-xia1,2,3,TANG Geng-guo4,ZHANG Yong-chun2,3,5,JING Xiang-mi4(1.Horticultural Research Institute,Shanghai Academy of Agricultural Science,Shanghai,201106,China;2.Flower Reasearch Institute,Shanghai Academy of Agricultural Science,Shanghai,201106,China;3.Shanghai Key Lab of Protected Horticutural Technology,Shanghai 201106,China;4.College of Forest Resources and Environment Nanjing Forestry University,Nanjing 210037,China;5.Forestry and Fruit Research Institute,Shanghai Academy of Agricultural Science,Shanghai 201106,China);Study on Optimization for ISSR Reaction System in Chimonanthus Praecox[J];Northern Horticulture;2007-10
10 WEI Xin1,WEI Yong-xiang1,WANG Ying2,LIU Cheng1,WANG Xing-dong1,DONG Wen-xuan3(1.Liaoning Fiuit Reasearch Institute,Liaoning,Xiongyao 115009;2.Horticultural College of,Jinlin Agricultural University,Changchun,Jilin 130118;3.College of Horticultural,Shenyang Agricultural Vniersity,Shenyang,Liaoning 110161);Optimization of the Inter-simple Sequence Repeat Reaction System and Its Verification in Ping'ou Hybrid Hazelnut[J];Northern Horticulture;2010-04
China Proceedings of conference Full-text Database 6 Hits
1 Wang Yongqing,Fu Yan,Yang Qin,Luo Nan,Deng Qunxian,and Yan Juan (College of Horticulture,Sichuan Agricultural University,Ya'an 625014);Analysis of 41 Eriobotrya Accessions Using ISSR Markers[A];[C];2011
2 LI Fang-ying~1 HUANG Yan-jing~2 WU Shao-hua~2 (1 College of Landscape Architecture,Fujian Agriculture and Forestry University,Fuzhou 350002; 2 College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002);Establishment and Optimization of ISSR System in Bougainvillea[A];[C];2010
3 FAN Yan~(1,2) LI Fang~1 ZHANG Xin-Quan~(1*) MA Xiao~1 (1.Department of Grassland Science Sichuan Agricultural University Sichuan Ya'an 625014 China 2.Chongqing Municipal Institute of Animal Husbandry Chongqing 400039 China);Genetic Diversity of Hemarthria compressa Detected by Inter-simple Sequence Repeat(ISSR) Molecular Marker[A];[C];2007
4 Liu Haiyan Zou Tiancai Zhou Hongying Hu Xuehua Lou Yilong (Guizhou Botanical Garden, Guiyang 550004;School of Geography and Biological Science, Guizhou Normal University, Guiyang 550001;Guiyang Bureau of Forestry and Landscaping,Guiyang 550004);Study on Introduction of Twenty Australian Endemic Ornamentals[A];[C];2006
5 Zou Tiancai Liu Haiyan (Guizhou Botanical Garden, Guizhou, Guiyang 550004);The Research and Innovative Development of Plants Ex-situ Conservation in Guizhou Botanical Garden[A];[C];2008
6 Zhou Hongying~(1,2) Liu Haiyan~(1*) Zhang Zhulin~1 Zou Tiancai~1 Zhu Li~(1,2) (1. Guizhou Botanical Garden, Guizhou Guiyang 550004 2. Forest School of Guizhou University, Guizhou Guiyang 550025);Investigation of Affected Plants by Freezing Disasters and Remedial Measures in Guizhou Botanical Garden[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 YANG Yu-Hong(College of Life Science and Resources Environment,Yichun University,Yichun,Jiangxi 336000,China);Induction Differentiation and Plant Regeneration of Camellia chekiangoleosa of mingyueshan[J];Anhui Agricultural Science Bulletin;2008-24
2 LI Hai-li et al(National Research Center of Floriculture Engineering,Beijing Forestry University,Beijing 100083);Establishment of the Optimum Reaction System for ISSR-PCR of Hemerocallis fulva L.[J];Journal of Anhui Agricultural Sciences;2008-12
3 WANG Li-ying et al(Zhejiang Forestry College,Lin′an,Zhejiang 311300 );Comparison of Leaf Gas Exchange and Chlorophyll Fluorescence Parameters in Three Species of Ilex[J];Journal of Anhui Agricultural Sciences;2008-17
4 JIA Wen-jie et al (College of Life Science,Nanchang University,Nanchang,Jiangxi 330031);Extraction of Genomic DNA and Optimization of ISSR Reaction Condition for Aquilaria sinensis(Lour.) Gilg[J];Journal of Anhui Agricultural Sciences;2008-24
5 CHAI Su-fen et al(Department of Life Sciences,Huizhou University,Huizhou,Guangdong 516007);Construction and Optimization of ISSR Reaction System of Medicinal Rutaceae Plants[J];Journal of Anhui Agricultural Sciences;2008-33
6 MA Li-ya et al (College of Life Sciences,Nanchang University, Nanchang,Jiangxi 330031);Establishment and Optimization of ISSR-PCR Reaction System for Cybidium goeringii[J];Journal of Anhui Agricultural Sciences;2008-34
7 ZHOU Li et al (College of Horticulture and Forestry Science,Huazhong Agricultural University,Wuhan,Hubei 430070);Establishment and Optimization of ISSR-PCR Reaction System for the Hybrid of Cymbidium cyperifolim var.szechuanicum and C.floribundum[J];Journal of Anhui Agricultural Sciences;2009-17
8 WEN Mei-xia et al(College of Landscape and Art,Jiangxi Agricultural University,Nanchang,Jiangxi 330045);Research on the Cutting Propagation Technique of Ilex rotunda[J];Journal of Anhui Agricultural Sciences;2010-18
9 ZHU Xiao-ting,LIN Xia-zhen(College of Landscape Architecture,Zhejiang Agricultural and Forestry University,Lin'an,Zhejiang 311300);The Effect of Chemical Reagent on Container Seedlings of Prunus zippeliana Miql[J];Northern Horticulture;2011-12
10 Li Genyou; Jin Shuihu; Lou Luhuan; Chen Zhenghai; Sun Mengjun.College of Life Sciense, Zhejiang Forestry College, Lin'an, 311300, P. R. China.;Flora amount and community of Chimonanthus praecox in Zhejiang Province, eastern China[J];Journal of Beijing Forestry University;2003-06
【Secondary References】
Chinese Journal Full-text Database 7 Hits
1 HUANG Bin-long;RUAN Shao-ning;MA Zhi-hui;CHEN Yu;HE Shi-bin;LIN Si-zu;College of Forestry,Fujian Agriculture and Forestry University;State Forestry Administration Engineering Research Center of Chinese Fir;College of Life Sciences,Fujian Agriculture and Forestry University;Zhangpu Zhongxi State-owned Forestry Farm;;The effects of different light mediums on the growth of container seedlings of Acacia cincinnata[J];Journal of Forest and Environment;2015-02
2 Xue Kena;Ke Huan;Chen Xiang;Li Xin;Xuan Zuying;Foshan Municipal Forestry Research Institute;;Effects of Different Fertilizers on Early Growth of Tutcheria championi[J];Protection Forest Science and Technology;2015-09
3 DONG Wen-yuan;FU Jian-sheng;DENG Shao-chun;LU Hua;XU Yan-fei;ZHU Feng;SHENG Wen-dong;Southwest Forestry University;;The impacts of different tending measures on growth of Manglietia grandis[J];China Forestry Science and Technology;2013-06
4 FU Jian-sheng;DONG Wen-yuan;LIU Xing-dong;ZHAO Min-yan;Southwest Forestry University;;Research Advances on Manglietia in China[J];Forest Inventory and Planning;2014-02
5 LI Ming-wei;YE Wei-yan;LIU Peng;LIU Hui-min;Southwest Forestry University;;Effects of different substrates on grafting seedling growth of grapefruit[J];Nonwood Forest Research;2014-03
6 CHEN Hai-yun1,CHEN Shao-yu1,2,NING De-lu1,WU Tao1,2,GENG Shu-xiang1, GU Li-ping1,BAI Ping1,LI Yong-jie1(1.Yunnan Academy of Forestry,Kunming Yunnan 650201,P.R.China;2.Yunnan Laboratory for Conservation of Rare,Endangered & Endemic Forest Plants,Public Key Laboratory of the State Forestry Administration;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants,Kunming Yunnan 650201,P.R.China);Establishment of RAPD-PCR Reaction System and Primer Selection[J];Journal of West China Forestry Science;2012-04
7 SHENG Qian-qian;WEN Bing;ZHU Zun-ling;Institute of Landscape Architecture,Nanjing Forestry University;College of Art and DesignNanjing Forestry University;;Effects of planting density on growth of Quercus rubra seedlings[J];Journal of Central South University of Forestry & Technology;2014-08
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Shuiming1,SONG Fengshun1,GONG Xuemei1,YANG Zhenjiang2,HUANG Yongfeng2,ZHU Liwu1(1.Department of Horticulture, Anhui Agricultural University,Hefei 230036;2.Administration Committee, Protection District of Dangshansu Pear Germplasm Resources);Isolation of Genomic DNA of Dangshansu Pearand Optimization of Its RAPD Analysis Condition[J];Journal of Anhui Agricultural University;2003-02
2 ZHANG Ji-yu~1,YUAN Qing-hua~2,ZHANG Wen-shu~2,LU Ting~3 (1. College of Pastoral Agriculture Science and Technology of Lanzhou University, Gansu Grassland Ecological Research Institute, Lanzhou 730020 China;2.Institute of Animal Science, CAAS,Beijing 100094 China; 3.Grassland College of Gansu Agriculture University, Lanzhou 730070, China);Genomic DNA Extraction and Optimizing RAPD Procedure of Lespedeza floribunda[J];Acta Agrestia Sinica;2004-03
3 LIU Jie,XIONG Yan wen,LIU Gong she *, QI Dong mei,LI Fang fang,WANG En hua (Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China);DNA extraction from different part of oil sunflower mature seeds for PCR analysis[J];Acta Botanica Boreali-occidentalia Sinica;2001-04
4 Huang Jin-pei Zou Qi-li( Department of Biology ( Guangxi Institute of Botany )Guangxi Teachers' College );KARYOTYPICAL OBSERVATIONS ON CHROMOSOMES OF CAMELLIA CHRYSANTHA(HU) TUYAMA[J];Guihaia;1982-01
5 Zou Qi-li Liang Sheng-yeh(Guangxi Institute of Botany) (Guangxi Institute of Forestry);POLLEN MORPHOLOGY OF CAMELLIA SECT. CHRYSANTHAE FROM GUANGXI[J];Guihaia;1984-03
6 Su Zong-ming (Guangxi Institute of Botany)Mo Xin-li (Guangxi Academy of Forest Exploration and Planning);GEOGRAPHIC DISTRIBUTION OF CAMELLIA SECTION CHRYSANTHA FROM CHINA[J];Guihaia;1988-01
7 Li Yingjie Zhao Shiqiang Yang Jinshen(Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050051);Review on Study and Commercial Development of Sunola Industry of Hebei Province[J];Journal of Hebei Agricultural Sciences;2002-02
8 Guan Ning (Research Institute of Wood Industry, Chinese Academy of Forestry);A STUDY ON CUTTING PROPERTIES OF WOOD OF KOREAN PINE AND MANCHURIAN ASH[J];Scientia Silvae Sinicae;1989-04
9 YU Yan CHEN Hai-shan GE Xue-jun*(South China Institute of Botany, the Chine se Academy of Sciences, Guangzhou 510650, China);Optimization of Experiment Conditions and PrimerScreening with ISSR Markers[J];Journal of Tropical and Subtropical Botany;2003-01
10 GE Yong-Qi, QIU Ying-Xiong, DING Bing-Yang, FU Cheng-Xin * Laboratory of Plant Systematic Evolution and Biodiversity, College of Life Sciences,Zhejiang University, Hangzhou 310029;An ISSR analysis on population genetic diversity of the relict plant Ginkgo biloba[J];Chinese Biodiversity;2003-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved