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《Journal of Anhui Agricultural Sciences》 2008-29
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Screening for High-yield Strain of Xylanase Produced by Engineered Pichia pastoris and Optimization of the Methanol Induced Conditions

JING Mei-zhen et al(College of Life Science,Henan Agricultural University,Henan,Zhengzhou 450002)  
[Objective] The aim of the study was to screen high-yield strain of xylanase by engineered Pichia pastoris and to optimize the methanol induced condition.[Method] Different concentrations of G418 on YPD plates were used to screen high-copy transformant,and then different concentrations of methanol were added into the medium at different time intervals to find the point of highest enzyme production.[Result] A high-yield strain No.16 was obtained which could bear 10.0 mg/ml of G418.When it was induced by 0.5% methanol at 30 ℃,the xylanase activity could reach the highest point of 826.2 IU/ml.And its specific activity reached 5 229.1 IU/mg.The enzyme production was high and stable when the methanol induced time interval was 12 h and the methanol concentration was 0.8%.[Conclusion] This strain was a methanol-dependent and high-yield one.
【Fund】: 河南省重点科技攻关项目(072102220001)
【CateGory Index】: Q78
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