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《Journal of Anhui Agricultural Sciences》 2008-29
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Screening for High-yield Strain of Xylanase Produced by Engineered Pichia pastoris and Optimization of the Methanol Induced Conditions

JING Mei-zhen et al(College of Life Science,Henan Agricultural University,Henan,Zhengzhou 450002)  
[Objective] The aim of the study was to screen high-yield strain of xylanase by engineered Pichia pastoris and to optimize the methanol induced condition.[Method] Different concentrations of G418 on YPD plates were used to screen high-copy transformant,and then different concentrations of methanol were added into the medium at different time intervals to find the point of highest enzyme production.[Result] A high-yield strain No.16 was obtained which could bear 10.0 mg/ml of G418.When it was induced by 0.5% methanol at 30 ℃,the xylanase activity could reach the highest point of 826.2 IU/ml.And its specific activity reached 5 229.1 IU/mg.The enzyme production was high and stable when the methanol induced time interval was 12 h and the methanol concentration was 0.8%.[Conclusion] This strain was a methanol-dependent and high-yield one.
【Fund】: 河南省重点科技攻关项目(072102220001)
【CateGory Index】: Q78
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Shi-chang et al(Department of Food and Bioengineering,Henan University of Science and Technology,Luoyang,Henan 471003);Effects of Low Energy N+ Ion,UV and ~(60)Co-γ on Xylanase Produced by Aspergillus niger[J];Journal of Anhui Agricultural Sciences;2007-17
2 LI Wei (College of Life Science,Yangtze University,Jingzhou,Hubei 434025,China)CHEN Song-lin Key Lab for Sustainable Utilization of Marine Fishery Resource,Ministry ofAgriculture,Yellow Sea Fisheries Research Institute,Chinese Academy ofFishery Science,Qingdao,Shandong 266071,China;Construction of Expression Vector of Turbot hepcidin Gene and Recombinant Expression in Pichia pastoris[J];Journal of Yangtze University(Natural Science Edition)Agricultural Science Volume;2008-04
3 LOU Kai,YU An-feng,XING Xin-hui(Department of Chemical Engineering,Tsinghua University,Beijing 100084,China);Production of Extremely Thermostable Xylanase in Recombinant Escherichia coli[J];The Chinese Journal of Process Engineering;2004-05
4 LI Shi-chang1,2,LI Ai-jiang,PAN Ren-rui2,YU Zeng-liang2*(1.College of Food & Bioengineering,Henan University of Science & Technology,Luoyang 471003,Henan,China;2.Institute of Plasma Physics,Chinese Academy of Sciences,Hefei 230031,Anhui,China);Mutation-screening in Xylanase High-yield Strains by Low-energy Ion Implantation Technique[J];Acta Laser Biology Sinica;2008-01
5 ZHANG Xiu-yan1,HE Guo-qing2,CHEN Qi-he2(1.Department of Food Safety and microbiology,Huazhong Agriculture University,Wuhan 430070,China; 2.Department of Food Science and Nutrition,Zhejiang University,Hangzhou 310029,China);Using of the Rational and Irrational Protein Design Strategies in Enzyme Engineering[J];Bulletin of Science and Technology;2007-02
6 OUYANG Jia1,2,WANG Xiang-ming2,ZHANG Qing2,YAN Ming2,XU Lin2(1.College of Chemical Engineering,Nanjing Forestry University,Nanjing 210037,China; 2.College of Life Science and Pharmacy,Nanjing University of Technology,Nanjing 210009,China);Secretory Expression of Trichoderma reesei XYN II Gene in Pichia pastoris[J];Chemistry and Industry of Forest Products;2007-05
7 LI Shi-Chang,GU Shao-Bin,LI Ai-Jiang (Food & Bioengineering College,Henan University of Science & Technology,Luoyang 471003,China);Study on Fermentation Condition with Xylanase Producing of Aspergillus Niger SP-1[J];Journal of Henan University of Science & Technology(Natural Science);2007-05
8 YAO Guo-yu1,LI Ning2,SHI Peng-jun2,CHEN Qiang1,YAO Bin2(1.Institute of Biochemistry and Molecule Biology,School of Life Science,Lanzhou University,Lanzhou 730000;2.Key Laboratory of Feed Biotechnology,Ministry of Agriculture,Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Cloning,Expression and Characterization of a Xylanase Gene,xynBS9,from Streptomyces sp.S9[J];Journal of Agricultural Science and Technology;2009-04
9 CUI Jin~1,KE Tao~2,MA Xiang-dong~2 (1.College of Life Sciences,Henan Agricultural University,Zhengzhou 450002 ,China;2.College of Life Sciences,Hubei University,Wuhan 430062,China);Studies on Expression of Xanthomonas campestris α-Amylase Gene in Pichia pastoris and Enzymatic Properties[J];Journal of Henan Agricultural University;2006-03
10 LIU Wei-Feng MAO Ai-Jun ZHU Ling-Xiang QIAO Yu YU Wei DONG Zhi-Yang**(Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China);Overexpression of Bacillus pumilus β-1,4-Xylanase Gene (xynA ) in Escherichia coli[J];Journal of Agricultural Biotechnology;2004-04
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Zhang Honglian Yao Bin Yuan Tiezheng Wang Yaru Cao Shishu Fan Yunliu(Feed Research Institute,Beijing 100081;Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081);Purification and Properties of 43 kD Xylanase XYNA from Streptomyces olivaceoviridis A1[J];Journal of Agricultural Biotechnology;2002-01
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