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《Journal of Anhui Agricultural Sciences》 2008-31
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Research on the Pollen Germination and Growth in vitro of Davidia involucrata

PENG Hong-li et al (Environmental Management College of China,Qinhuangdao,Hebei 066004)  
[Objective] The causes and mechanism for Davidia involucrata endangered were revealed so as to provide the reference for its pollen preservation and cross breeding.[Method]The pollen germination and pollen tube growth of Davidia involucrata were tested with one way ANOVA and multiple-comparison.[Results] The results indicated that the pollen germination and pollen tube growth would be enhanced when the concentration of sucrose,boric scid,agar in the culture medium were within a certain range.If the concentrations were too high or low,the pollen germination and pollen tube growth would be inhibited.The effect of IAA on pollen germination and pollen tube growth was in significant.The optimum temperature affecting pollen germination and pollen tube was from 20 to 25 ℃.Humidity had big effect on pollen fertility.When atmosphere was dry,its germination ability was reduced.But when atmosphere was too humid,the pollen drank too much water and broke,and its germination ability was reduced,too.[Conclusion] Davidia involucrata pollen germination and pollen tube growth varied with the change of medium component and culture conditions and its pollen germination and pollen tube growth required not only an appropriate concentration of the components,but also had the suitable culture condition.
【Fund】: 国家自然科学基金资助项目(30370149 39770134)
【CateGory Index】: S685.99
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