Study on Effective Components Extraction and Antioxidant Capacities of Gynostemma pentaphyllum Makino
LIU Xin-yan et al (School of Life Science,Nantong University,Nantong,Jiangsu 226007)
[Objective] The research aimed to compare antioxidant capacities of two kinds of extracts in Gynostemma pentaphyllum Makino.[Method] Scavenging effects on free oxygen radicals of the active fractions,which were extracted with different solvents from two kinds of Gynostemma pentaphyllum Makino,were studied and the total flavones of the extracts were assayed.[Result] The extracting results were optimal when the solvent was 100% ethanol at the temperature of 90 ℃ for 2 hours.Initial appraisal indicated that there were flavones and other flavonoids in the extracts of Gynostemma.100% ethanol extracts of two kinds of Gynostemma had significant effects to the others,and the scavenging effect of 100% ethanol extract of Gynostemma Ⅰ was more fantastic.After 30 minutes' reaction with the concentration at 0.6 mg/ml,the scavenging activity of the extract could reach 51.20%.Scavenging effects on free oxygen radicals of the extracts of 100% ethanol,75% ethanol and water were all stronger than that of vitamin C.[Conclusion] Antioxidant capacity of wild Gynostemma extract was slightly higher than that of artificial cultivation of Gynostemma,but the difference was not big.It showed that the environment in Nantong was suitable for cultivation Gynostemma.