Preliminary Research on the Establishment of Multiplication System of Rough Gentian
AN Yong-hui et al(Changchun Normal College,Changchun,Jilin 130032)
[Objective] The bud induction and its multiplication of rough gentian were researched in the experiment.[Method] The healthy rough gentian stem being taken as explants,the high-efficiency multiplication system was set up through the orthogonal design including 3 media(MS,MB and N6) and 3 kinds of plant growth regulators(IBA,6-BA and GA).[Results] The results showed that 0.1% HgCl2 was the best sterilized agent.When the fundamental medium was the MB(macro-element of MS + trace element and organic material of B5 + Fe) and IBA was 2.0 mg/L,6-BA was 0.5 mg/L,GA was 2.0 mg/L,the rate of shoot induction and multiplication of rough gentian were highest.[Conclusion] The results could be used in tissue culture and rapid multiplication of rough gentian.
【CateGory Index】： S681.9