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《Journal of Anhui Agricultural Sciences》 2008-32
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Study on Establishment of Regeneration System of Cymbidium

YANG Lu-jun et al(Zhengzhou Institute of Science from Agriculture and Forest in Henan Province,Zhengzhou,Henan 450005)  
[Objective] The research aimed to study and establish the regeneration system of Cymbidium.[Result] 75% alcohol and 0.1% were used to surface sterilization.The MS was used as basic culture medium.L9(33) orthogonal design was chose to optimize the protocorm-like body proliferation.The proliferation coefficient and the seedling differentiation rate after subcultured 40 d were chose as the index.[Result] At in vitro propagation of the regeneration system built of cymbidium,axially bud was more easily started as explants.MS agar medium supplemented with 6-BA 1.0 mg/L+NAA 0.1 mg/L +AC 0.1 g/L was the optimal medium for the induction of protocor-like body.1/2 MS+6-BA 1.0 mg/L +NAA 0.1 mg/L was the best medium of protocor-like body multiplying and seedling differentiation.After seedling differentiation,in 1/2 MS medium supplemented with 1/2 MS+NAA 1.0 mg/L +GA3 1.0 mg/L + banana mud 150 g/L,the rooting and strong sprout of rootless bud could be accomplished efficiently and fast.[Conclusion] The study provided the basis for scale productin.
【Fund】: 河南省科技厅重点攻关项目(0623010500)
【CateGory Index】: S682.31
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