Full-Text Search:
Home|About CNKI|User Service|中文
Add to Favorite Get Latest Update

Isolation of Chlorella vulgaris and Its DNA Extraction Methods

REN Xue-yan et al(College of Life Sciences,Shihezi University,Shihezi,Xinjiang 832003)  
[Objective] The aim of this study is to isolate Chlorella vulgaris(chlorella) and extract its genomic DNA.[Method] Both the dilution method and drip method were employed to isolate chlorella from lake water samples;the conditions for culturing chlorella were optimized and its genomic DNA was extracted by improved CTAB method and SDS method.[Result] The proper conditions for chlorella culture were as following: temperature 20-25 ℃,illumination 4.39-5.86 W/m2 and rotational speed 100-150 r/min;improved CTAB method was suitable for extracting genomic DNA from chlorella.[Conclusion] The result is helpful for studying the chlorella at molecular level and promote the exploitation and utilization of chlorella resources.
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©CNKI All Rights Reserved