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《Journal of Anhui Agricultural Sciences》 2008-32
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Isolation of Chlorella vulgaris and Its DNA Extraction Methods

REN Xue-yan et al(College of Life Sciences,Shihezi University,Shihezi,Xinjiang 832003)  
[Objective] The aim of this study is to isolate Chlorella vulgaris(chlorella) and extract its genomic DNA.[Method] Both the dilution method and drip method were employed to isolate chlorella from lake water samples;the conditions for culturing chlorella were optimized and its genomic DNA was extracted by improved CTAB method and SDS method.[Result] The proper conditions for chlorella culture were as following: temperature 20-25 ℃,illumination 4.39-5.86 W/m2 and rotational speed 100-150 r/min;improved CTAB method was suitable for extracting genomic DNA from chlorella.[Conclusion] The result is helpful for studying the chlorella at molecular level and promote the exploitation and utilization of chlorella resources.
【Fund】: 石河子大学高层次人才启动基金(07002-500002061401)
【CateGory Index】: Q943.2
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Chinese Journal Full-text Database 10 Hits
1 YE Lin-chao,YE Jun-an,XU Guo-zhong,LIU Jian-xin(College of Animal Science,Zhejiang University,Hangzhou 310029,China);Effects of Nitrogen Sources on Growth of Alga Chlorella vulgaris[J];Fisheries Science;2007-06
2 SUN Li-qin,REN Li-hong,WANG Chang-hai (College of Ocean,Yantai University,Yantai 264005,China);Semi-continuous Culture of Alga Nitzschia closterium in a Flat Plate Photobioreactor[J];Fisheries Science;2009-05
3 WANG Ping~1,WANG Jinhua~2(1.Marine Science and Technology College of Zhejiang Ocean University,Zhoushan 316004,China;2.Natural Resources College of Southwest Forestry Institute,Yunnan Kunming 650224,China);A Study on Iodine Accumulation of Chlorella vulgaris[J];Guangdong Trace Elements Science;2006-08
4 Yi Tingqiang,et al (Chemical Engineering College of Sichuan University, Chengdu 610065);Research Progress of Cultivation of Marine Microalgae and Photo-bioreactor[J];Chemical Engineering Design;2008-03
5 HU Gui-kun ZHANG Qing-tian (College of Marine Science and Engineering,Tianjin University of Science & Technology,Tianjin 300457,China);Effects of NH_4 Cl and NaH_2 PO_4 on the Growth of Chlorella[J];Journal of Salt and Chemical Industry;2006-05
6 LV Rong,ZHANG Bo(Marine Science & Engineering School,Tianjin Universitiy of Science& Technology,Tianjin 300457,China);Effects of Selenium Concentration on the Growth and Bioaccumulation of Chlorella Pyrenoidosa[J];Journal of Salt and Chemical Industry;2008-04
7 WANG Heng-qiang,KONG Qing-jun,REN Xue-yan,ZHAN Dong-xia,ZHANG Hai-li College of Life Sciences,Shihezi University,Shihezi 832003;Isolation of Chlorella vulgaris and Its DNA Extraction Methods[J];农业科学与技术(英文版);2008-04
8 YE Lin-Chao,YE Jun-An,XU Guo-Zhong,LIU Jian-Xin(College of Animal Science,Zhejiang University,Hangzhou,310029);ANIMAL PROTEIN ENZYMATIC HYDROLYSIS,N,AND P SOURCES:COMBINATION EFFECT ON GROWTH AND BIOCHEMICAL PARAMETERS OF CHLORELLA VULGARIS[J];Oceanologia Et Limnologia Sinica;2009-02
9 ZHU Yi feng1 WU Song jie2 GUO Xiao qiang1(1Department of Fisheries, Ningbo University, 315211)(2Ningbo Fisheries Research Institute, 315010);EFFECTS OF NITROGEN, PHOSPHORUS CONCENTRATION AND RENEWAL RATE ON RECOVERY YIELDS OF Phaeodactylum tricornutum IN SEMICONTINUOUS CULTURES[J];Marine Sciences;2000-11
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