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《Journal of Anhui Agricultural Sciences》 2008-32
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Cloning and Analysis of BADH Gene from Helianthus annuus

YI Le-fei et al(School of Marine Science & Technology,Huaihai Institute of Technology,Lianyungang,Jiangsu 222005)  
[Objective]The research was to provide the theory and practical basis for the utilization of BADH Gene from Helianthus annuus.[Method]Partial sequence of sunflower BADH cDNA was obtained by the reverse transcription polymerase chain reaction(RT-PCR) with silico cloning technique.[Results]The partial gene sequence was obtained with RT-PCR.The PCR production was electrophoresed in 1.0% agarose gel,and a specific band of about 1 584 bp length was observed,which size was similar to designed size.Sequence comparison of sequencing result revealed 99.22% nucleotide sequence identity with in silico cloning result.Protein deduced by sequencing result contained conserved amino acids residue and sequences closely related to function of enzyme,and possessed high amino acid sequence identity with BADH form other organism.[Conclusion]The partial sunflower BADH gene sequence was successfully cloned.
【Fund】: 淮海工学院自然科学基金(Z2007035);; 淮海工学院引进人才科研启动基金(KK01073)资助
【CateGory Index】: S565.5
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