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《Journal of Anhui Agricultural Sciences》 2008-32
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Extraction and Immuno-identification of Outer Membrane Protein from Vibrio parahaemolyticus

JU Er-yi et al(College of Food Science,Shanghai Fisheries University,Shanghai 201303)  
[Objective] The aim was to lay the foundation for the further determination on the mutual specific antigen and the protective antigen of the pathogenic V.parahaemolyticus.[Method] The pathogenicity of the 5 strains of V.parahaemolyticus to mouse were studied by the mice virulence experiments,the SDS-PAGE map of the outer membrane protein extracted under the different medium and culture time was compared and the immunogenicity of the V.parahaemolyticus strain was studied by the western blotting analysis.[Result] The 5 strains of V.parahaemolyticus had different pathogenicity to mouse obviously and could form obvious hemolytic cycle on rabbit blood plate,being TDH positive strain.The polyclonal antibody obtained by strain immunity all had no cross reaction with other experimental strains except V.parahaemolyticus strain.Western blotting analysis showed that the polyclonal antibody could produce positive reaction with the 5 strains of pathogenic V.parahaemolyticus to a different degree and the extracted outer membrane protein had better immunogenicity.[Conclusion] The research provided the theoretical basis for the preparation of the vaccine to prevent the disease caused by the V.parahaemolyticus effectively.
【Fund】: 上海市科委重点资助项目(T1102)
【CateGory Index】: R378
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Chinese Journal Full-text Database 4 Hits
1 ZHANG Wei-ni,ZHOU Li,XING Jing,ZHAN Wen-bin (The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China);Antigenicity of outer membrane proteins of a pathogenic Vibrio strain,Vibrio alginolyticus[J];Journal of Fishery Sciences of China;2007-03
2 Zhang Xiao hua Xu Huai shu (Ocean University of QingDao, Qingdao 266003) Peter Robertson Brian Austin (Heriot-Watt University, Edinburgh, United Kingdom);ANTIGENICITY OF OUTER MEMBRANE PROTEINS OF VIBRIO PARAHAEMOLYTICUS[J];JOURNAL OF FISHERY SCIENCES OF CHINA;1997-04
3 HUANG Zhi_jian, HE Jian_guo (School of Life Sciences,Sun Yat_sen(Zhongshan) University, Guangzhou 510275, China);Isolation, Identification and Pathogenicity of the Bacterial Pathogen in Epinephlus fario[J];Acta Scientiarum Naturalium Universitatis Sunyatseni;2002-05
4 Liu Xiumei (National Institute for Nutrition and Food Safety, China CDC, Beijing 100050);Studies on the techniques for the monitoring and controlling foodborne illness[J];Chinese Journal of Food Hygiene;2004-01
Chinese Journal Full-text Database 10 Hits
1 TANG Bao Gui CHEN Gang ZHANG Jian Dong YE Fu Liang (Fisheries College, Zhanjiang Ocean University, Zhanjiang 524025, China);Effects of pH,Amylum Concentration and Temporary-keeping Salinity on the Activity of Amylase in the Digestive Tract of Lautianus erythopterus[J];Chinese Journal of Zoology;2004-02
2 HUANG Rui-fang(Fujian Fisheries Research Institute,Xiamen 361012,China);Vibrio alginolyticus in the Grouper, Epinephelus coioides[J];Fisheries Science;2005-06
3 ZHENG Qiu-yue1,2,CAO Ji-juan1,WANG Qiu-yan1,XU Jun-yi1,XU Yang1,FU Jun-fan2(1.Liaoning Entry-Exit Inspection & Quarantine Bureau,Dalian 116001,China;2.Shenyang Agricultural University,Shenyang 110161,China);Detection of Vibrio alginolyticus with PCR-DHPLC in Fishery Products[J];Fisheries Science;2009-03
4 WAN Wen-ju1,2,3 WANG Ji-ting3, SHI Cun-bin1, WU Shu-qin1 (1.Pearl River Fisheries Research Institute,Chinese Academy of Fishery Science,Guangzhou 510380,China;2.Department of Basic Medicine,Taishan Medical University,Tai'an 271000,China;3.College of Animal Science & Technology,Shandong Agricultural University,Tai'an 271018,China);Gene expression of HSP70 in green swordtail Xiphophorus helleri exposed to Vibrio alginolyticus[J];Journal of Dalian Fisheries University;2007-05
5 WANG Kai-yu,HE Min (Research Center of Aquatic Animal′s Disease,Sichuan Agricultural University,Ya′an,Sichuan 625014);Application Progress of Pathologic Technology in Aquatic Animal Disease[J];Freshwater Fisheries;2007-05
6 Luo Zhaofei1 Zhang Weiyu1 Yang Desheng2(1. The faculty of animal science and technology,Guangxi university,Nanning 530022;2. Center for prevent and control the animal diseases in Fujian province,Fuzhou 350003);Detection of Proteus mirabilis in animal-derived food by Polymerase Chain Reaction[J];Fujian Journal of Animal Husbandry and Veterinary Medicine;2008-01
7 GONG Yan-qing~1,CHEN Xin-zhong~(1,2),WANG Jun~2,SU Ya-ling~3,YU Xiu-xia~3,HUANG Li-sha~3,SU Yong-quan~2(1.Xiamen Entry-Exit Inspection and Quarantine Bureau,Xiamen,Fujian 361012,China;2.College of Oceanography and Environment,Xiamen University,Xiamen,Fujian 361005,China;3.Xiamen Aquaculture Technical Service Station,Xiamen,Fujian 361005,China);Investigation on the epidemic of fulminant infectious disease of cultured rockfish in the south Fujian[J];;2006-05
8 TIAN Ding1,2,LIN Tian-long2,XU Bin-fu2,GONG Hui2(1.College of Life Sciences Fujian Normal University,Fuzhou,Fujian 350007,China;2.Biotechnology Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China);Antigenicity and identification of Vibrio vulnificus outer membrane proteins[J];Fujian Journal of Agricultural Sciences;2008-04
9 DU Yu-ping,CHEN Qing,KE Xue-mei,et al.Department of Epidemiology,School of Public Health and Tropical Medicine,Southern Medical University,Guangzhou 510515,China;Preparation of anti-thermolabile hemolysin of vibrio parahaemolyticus polyclonal antibodies and detection by the ELISA double sandwich method[J];South China Journal of Preventive Medicine;2007-01
10 Yu Ying,Ning Xi-bin (Food College,Shanghai Fisheries University,Shanghai 200090,China);The Risk Assessment of Vibrio parahaemolyticus in Prawn[J];Modern Food Science and Technology;2006-03
China Proceedings of conference Full-text Database 1 Hits
1 ;A Design on the Frame of food safety forecasting & pre-warning information system in China[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 DONG Chuan-Fu 1,LIN Tian-Long 1,GONG Hui 1,OU-YANG Sui-Dong 1 and YANG Su 2(1.Institute of Animal Husbandry and Veterinary Medicine;2.Center laboratory,Fujian Academy of Agricultural Sciences,Fuzhou,China 350003);MAJOR OUTER MEMBRANE PROTEIN(MOMP)OF AEROMONAS HYDROPHILA INDUCED PROTECTIVE IMMUNITY TO EUROPEAN EELS(ANGUILLIA ANGUILLIA)[J];Acta Hydrobiologica Sinica;2005-03
2 Zhang Xiaohua Robertson P. Austin B. Xu Huaishu(Ocean University of Qingdao, Qingdao 266003) (Heriot-Watt University, Edinburgh, United Kingdom);COMPARISON OF OUTER MEMBRANE PROTEIN PROFILES OF VIBRIO SP.[J];Acta Microbiologica Sinica;1997-06
3 Liu Xiumei,Chen Yan,Wang Xiaoying,Ji Rong National Institute of Nutrition and Food Safety,Chinese Center for Di sease Control and Prevention,Beijing 100050,China;Foodborne disease outbreaks in China from 1992 to 2001 national foodborne disease surveillance system[J];Journal of Hygiene Research;2004-06
4 CUI Yu-bao.Division of Medical Entomology,Medical College,Anhui University of Sciene and Technology, Huainan, 232001);Investigation on dust mite allergy in students[J];Chinese Journal of School Doctor;2003-01
5 DONG Chuan-fu,LIN Tian-long,XU Bin-fu,GONG Hui,LIN Neng-feng (Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350003,China);Electrophoretic and immunobot analysis of the major outer membrane protein (MOMP) and polysaccharides antigen(PSA) of Vibrioparaheamolyticus and Vibrio alginolticus[J];Chinese Journal of Zoonoses;2004-07
6 WU Hou bo, PAN Jin pei (South China Sea Institute of Oceanology, Chinese Academy of Sciences,Guangzhou 510301, China);Progress in studies of vibriosis in aquaculture[J];Journal of Fishery Sciences of China;2001-01
7 ZHOU Li, LIU Hongming, ZHAN Wenbin, SONG Weibo cean University of China, Qingdao 266003, China );Isolation and characteristics of major outer membrane proteins of aquatic pathogens Vibrio anguillarum and Vibrio alginolyticus[J];Journal of Fishery Sciences of China;2003-01
8 ZHANG Wei-ni,ZHOU Li,XING Jing,ZHAN Wen-bin(The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China);Isolation and identification of pathogen SR1 associated with swollen abdomen of cultured turbot (Scophthalmus maximus)[J];Journal of Fishery Sciences of China;2006-04
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