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《Journal of Anhui Agricultural Sciences》 2008-32
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Extraction and Immuno-identification of Outer Membrane Protein from Vibrio parahaemolyticus

JU Er-yi et al(College of Food Science,Shanghai Fisheries University,Shanghai 201303)  
[Objective] The aim was to lay the foundation for the further determination on the mutual specific antigen and the protective antigen of the pathogenic V.parahaemolyticus.[Method] The pathogenicity of the 5 strains of V.parahaemolyticus to mouse were studied by the mice virulence experiments,the SDS-PAGE map of the outer membrane protein extracted under the different medium and culture time was compared and the immunogenicity of the V.parahaemolyticus strain was studied by the western blotting analysis.[Result] The 5 strains of V.parahaemolyticus had different pathogenicity to mouse obviously and could form obvious hemolytic cycle on rabbit blood plate,being TDH positive strain.The polyclonal antibody obtained by strain immunity all had no cross reaction with other experimental strains except V.parahaemolyticus strain.Western blotting analysis showed that the polyclonal antibody could produce positive reaction with the 5 strains of pathogenic V.parahaemolyticus to a different degree and the extracted outer membrane protein had better immunogenicity.[Conclusion] The research provided the theoretical basis for the preparation of the vaccine to prevent the disease caused by the V.parahaemolyticus effectively.
【Fund】: 上海市科委重点资助项目(T1102)
【CateGory Index】: R378
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