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《Journal of Anhui Agricultural Sciences》 2008-35
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Extraction of Flavonoids from Cottony Jujube Leaves and Its Content Determination by Voltammetry

LUO Ji-wen et al(Department of Chemistry and Biology,Yulin Normal College,Yulin,Guangxi 537000)  
[Objective]The aim was to study the optimum method on the extraction and determination of flavonoids from cottony jujube leaves.[Method] With fresh leaves of cottony jujube as the material,their flavonoids were extracted by ethanol,the effects of ethanol concn.extracting time and extracting temperature on extraction amount of flavonoids were studied.After the differential pulse voltammetry curve was drew with the rutin standard solution,the flavonoids content in extract was determined by differential pulse voltammetry in phosphate buffer.[Result] When the ethanol was 70%,the extracting temperature was 80 ℃ and extracting time was 4 h,the extraction amount of flavonoids was the highest.The differential pulse voltammetry by which the flavonoids content was determined had the characters of simple operation,good sensitivity and high detection limit.When the pH value of phosphate solution was 4.5,the detection limit of flavonoids was 3.0×10-7 mol/L.[Conclusion] The optimum extracting condition for flavonoids from cottony jujube leaves was that using ethanol to extract flavonoids for 4 h at 80 ℃.The detection effect on flavonoids content by differential pulse voltammetry was better.
【Fund】: 广西教育厅科研基金资助项目(200507218)
【CateGory Index】: S665.1
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