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《Journal of Anhui Agricultural Sciences》 2008-35
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Establishment of Axillary Buds Regeneration System of Cymbidium hybridum

CHEN Zhi-Hua et al(Sanming Institute of Agricultural Science,Sanming,Fujian 365509)  
[Objective]The purpose was to establish regeneration system of Cymbidium hybridum axillary buds.[Method]With axillary buds of Cymbidium hybridum as experimental materials,the optimal conditions of Cymbidium hybridum axillary buds regeneration were researched.[Result]The results indicated that the optimum medium of Cymbidium hybridum axillary buds regeneration was B5+6-BA 3.0-4.0 mg/L+KT 0.5 mg/L+NAA 0.1 mg/L+AD 2.0 mg/L+YE 100.0 mg/L.Radication medium adding 2.0 g/L AC and 0.3 mg/L NAA would get well effect,radication rate was 90% and root number reached 3-4 items;whether adding 6-BA had not effect on radication rate.[Conclusion]This experiment used axillary buds regeneration model,which have higher genetic stability.
【CateGory Index】: S682.31
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