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《Journal of Anhui Agricultural Sciences》 2008-36
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Analysis on the Content of Taxol in Different Collection Season of Taxus cuspidata Branches and Leaves

LI Feng-wei et al(Department of Chemical and Biological Engineering,Yancheng Institute of Technology,Yancheng,Jiangsu 224051)  
[Objective] The research aimed to study the content of taxol from Taxus cuspidata branches and leaves in different collection season to confirm the best collection period,and provide the theoretical direction for realizing the industrialization production of artificial cultivate Taxus cuspidata.[Method] The separation was performed by RP-HPLC,TOSOH C18 column(4.6 mm×150 mm,5 μm),eluted with acetonitrile,0.2 mol/L ammonium acetate and glacial acetic acid(41∶59∶0.2) as mobile phase with the rate of 1.0 ml/min at 25 ℃,the wavelength for measurement was 230 nm.[Result] The highest content period of wild and bionic Taxus cuspidata was in Four and May.[Conclusion] This method is simple,rapid and accurate,and it can be used to determine taxol in Taxus cuspidata.
【CateGory Index】: S791.49
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