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《Journal of Anhui Agricultural Sciences》 2009-01
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Preliminary Studies on ITS Sequences of nrDNA from Rhodiola alsia

GAO Qing-bo et al (Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,Qinghai 810001)  
[Objective] The study aimed to analyze the ITS sequences of nrDNA from Rhodiola alisa and investigate the difference of evolution rate between nrDNA and trnS-trnG and rpl20-rps12 sequences of cpDNA(chloroplast DNA).[Method]Total DNA was extracted from silica-dried leaves of R.alsia by using modified CTAB method.With the extracted DNA as template,nrDNA ITS region was amplified,then purified and sequenced.In addition,the yielded ITS sequences were also compared with the known trnS-trnG and rpl20-rps12 sequences of cpDNA from R.alsia.[Result]The ITS sequence of nrDNA from R.alsia was 701 bp in length,of which 13 variable sites were found with a percentage of 1.85%.Of the 13 variable sites,8 were caused by point mutations,5 were the results of insertions or deletions. The(A+T) content and(G+C) content were 46.9% and 53.1%,respectively.The nucleotide diversity(π) was 0.004 27.[Conclusion]The ITS region of nrDNA from R.alsia was more conservative and evolved more slowly than the trnStrnG and rpl20-rps12 sequences of its cpDNA.
【Fund】: 国家自然科学基金(30170066 30370284);; 国家重点基础研究发展计划项目(2008CB117013);; 瑞典国家自然科学基金(IFSD4258-1)
【CateGory Index】: S567.239
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