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《Journal of Anhui Agricultural Sciences》 2009-01
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Preliminary Studies on ITS Sequences of nrDNA from Rhodiola alsia

GAO Qing-bo et al (Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,Qinghai 810001)  
[Objective] The study aimed to analyze the ITS sequences of nrDNA from Rhodiola alisa and investigate the difference of evolution rate between nrDNA and trnS-trnG and rpl20-rps12 sequences of cpDNA(chloroplast DNA).[Method]Total DNA was extracted from silica-dried leaves of R.alsia by using modified CTAB method.With the extracted DNA as template,nrDNA ITS region was amplified,then purified and sequenced.In addition,the yielded ITS sequences were also compared with the known trnS-trnG and rpl20-rps12 sequences of cpDNA from R.alsia.[Result]The ITS sequence of nrDNA from R.alsia was 701 bp in length,of which 13 variable sites were found with a percentage of 1.85%.Of the 13 variable sites,8 were caused by point mutations,5 were the results of insertions or deletions. The(A+T) content and(G+C) content were 46.9% and 53.1%,respectively.The nucleotide diversity(π) was 0.004 27.[Conclusion]The ITS region of nrDNA from R.alsia was more conservative and evolved more slowly than the trnStrnG and rpl20-rps12 sequences of its cpDNA.
【Fund】: 国家自然科学基金(30170066 30370284);; 国家重点基础研究发展计划项目(2008CB117013);; 瑞典国家自然科学基金(IFSD4258-1)
【CateGory Index】: S567.239
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 ZHANG De-jun et al(Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences, Xining,Qinghai 810001);An High-effective Method of Extracting Genomic DNA from Saxifragaceae Plants[J];Journal of Anhui Agricultural Sciences;2008-16
2 Zhang Chi Hu Hongjun Li Zhongkui Li Yeguang Zhong Yang (Wuhan Institute of Botany,The Chinese Academy of Sciences Wuhan 430074);PHYLOGENETIC ANALYSIS ON GENUS CHLAMYDOMONAS BASED ON MORPHOLOGICAL CHARACTERS AND nrDNA ITS SEQUENCE[J];JOURNAL OF WUHAN BOTANICAL RESEARCH;2000-03
3 TIAN Xin,LI De Zhu (Kunming Institute of Botany,Chinese Academy of Sciences, Kunming 650204,China);Application of DNA Sequences in Plant Phylogenetic Study[J];Acta Botanica Yunnanica;2002-02
4 YUAN Chang_chun 1,SHI Su_hua 1,ZHONG Yang 2, GONG Xun 1(1. School of Life Sciences, Sun Yat_sen(Zhongshan) University, Guangzhou 510275, China; 2. School of Life Sciences, Fudan University, Shanghai 200433, China);A Reevaluation of the Taxonomic Position of the Genus Craigia Based on the nrDNA ITS Sequence[J];Acta Scientiarum Naturalium Universitatis Sunyatseni;2002-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 PU Xiang et al (College of Life Sciences,Southwest Jiaotong University,Chengdu,Sichuan 610031);Research Advance on Saxifraga stolonifera[J];Journal of Anhui Agricultural Sciences;2009-31
2 REN Zhi-long et al(Department of Biological Engineering,Baotou Vocational and Technical College of Light Industry,Baotou,Inner Mongolia 014023);Study on Improvement of Extraction Method of Total DNA from Astragalus membranaceus var.mongholicus[J];Journal of Anhui Agricultural Sciences;2009-36
3 MU Shao-hua et al (SFA Key Laboratory of Bamboo and Rattan Science and Technology,International Center for Bamboo and Rattan Beijing 100102);Genetic Diversity of Indocalamus Determined by Chloroplast DNA Sequence[J];Journal of Anhui Agricultural Sciences;2010-23
4 ZHANG De-jun et al(Medical College,Qinghai University,Xining,Qinghai 810001);Comparison of Chloroplast DNA psbA-trnH Sequences between Wild and Cultivated Gentiana straminea Maxim.in Qinghai[J];Journal of Anhui Agricultural Sciences;2011-10
5 BAI Rui1,HE Cong-fen1,DONG Yin-mao1,LI Lu-bin2,LIU Lei1,PENG Zhen-hua2(1.Beijing Key Lab of Plant Resources Research and Development,College of Chemistry and Environmental Engineering,Beijing Technology and Business University,Beijing 100037,China;2.Key Lab of Tree Breeding and Cultivation,State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China);A Preliminary Analysis of Phylogenetic Relationships in Orchidaceae Based on cp rRNA ITS Sequence Data[J];Northern Horticulture;2008-01
6 CUI Bin-bin1; LI Yun1;JIN Xiao-jie1; FENG Hui2. 1 Key Laboratory for Genetic and Breeding in Forest Trees and Ornamental Plants,Ministry of Education, Beijing Forestry University,100083, P. R. China;2 Beijing Institute of Landscape Architecture, 100102, P. R. China.;Genetic characters and polymorphism of chloroplast and mitochondrial DNA in white poplars.[J];Journal of Beijing Forestry University;2006-06
7 YANG Li-en~(1,2),JIN Jie~1,ZHU Jian-yi~1,HAN Xiao-lei~1,MU Xin-wu~(1,3),YAO Chun-yan~(1,3) XU Pu~1 (1.College of Biology and Food Engineering,Changshu Institute of Technology,Changshu 215500,China; 2.Department of Cell Biology,Suzhou University,Suzhou 215123,China; 3.College of Life Science,Nanjing Normal University,Nanjing 210046,China);Taxonomy of Porphyra(Rhodophyta,Bangiales) based on rbcL Sequences from the thallus[J];Journal of Changshu Institute of Technology;2009-08
8 HUANG Wen-da1,2,ZHAO Xue-yong1,ZHAO Xin1,ZHAO Ha-lin1,LIAN Jie1,2,WANG Shao-kum1,2(1.Cold and Arid Regions Environmental and Engineering Research Institute,Chinese Academy of Sciences,Gansu Lanzhou,730000,China;2.Graduate University of Chinese Academy of Science,Beijing 100039,China);Application of molecular markers in population genetics[J];Pratacultural Science;2010-11
9 ZHANG Li~(1,2),ZHOU Yong-hong~(1,2),ZHENG You-liang~(1,2),LIU Shi-gui~3(1.Triticeae Research Institute,Sichuan Agricultural University,Dujiangyan 611830,China;2.Key Laboratory of Crop Genetic Resources and Improvement,Ministry of Education,Sichuan Agricultural University,Ya'an 625014,China;3.College of Life Science,Sichuan University,Chendu 610064,China);PCR-RFLP analysis of plasmon in Kengyilia populations[J];Acta Prataculturae Sinica;2006-03
10 XIE Xin ming 1, ZHANG Shou zhou 2, LI Yong 2,WU Hong 1* , LI Bing tao 1 (1 Institute of Botany, South China Agricultural University, Guangzhou 510642, China; 2 Shenzhen Fairy Lake Botanical Garden, Shenzhen,Guangdong 518004, China);Systematic Positions of Pterospermeae (Sterculiaceae) on the Basis of DNA Sequence Data[J];Acta Botanica Boreali-occidentalia Sinica;2005-04
China Proceedings of conference Full-text Database 3 Hits
1 MU Shao-hua~1 SUN Zhen-yuan~2 PENG Zhen-hua~2 GAO Zhi-min~1 GUO Qi-rong~1 (1 International Center for Bamboo and Rattan,Beijing 100102;2 Research Institute of Forestry, Chinese Academy of Forestry,Beijing 100091);Genetic Diversity of Iris Determined by Chloroplast DNA Sequence[A];[C];2010
2 Mu Shaohua Gao Zhimin Guo Qirong Li Xueping Ma Yanjun (SFA Key Laboratory of Bamboo and Rattan Science and Technology, International Center for Bamboo and Rattan Beijing 100102);Genetic diversity of Indocalamus determined by chloroplast DNA sequence[A];[C];2009
3 GAO Jinguo,TIAN Xingjun,CHEN Bin,ZHU Jing,YANG Changlin, LI Zhongqi and SONG Fuqiang (School of Life Science,Nanjing University,Nanjing 210093);Application of DNA Molecular Markers in Plant Phylogeny Study[A];[C];2002
【Secondary Citations】
Chinese Journal Full-text Database 9 Hits
1 WANG Yan 1 LI Shaoshan 2 LIU Songhao 2 (1 School of Life Science and Technology, Jinan University , Guangzhou 510632) (2.Institute of Laser Life Science, South China Normal University, Guangzhou 510631);Quick Preparation of Total DNA from Plants[J];ACTA LASER BIOLOGY SINICA;2000-01
2 JIN Xiao-Ling 1,2 WU YUN Ta-Na 2 ZHANG Zhi-Jun 2 HE Ping 2,3 (1.Zhejiang Normal University, Jinhua 321004) (2.The Key Laboratory of Non-timber Breeding and Cultivation of National Forestry Ministry,Center-South Forestry University,Zhuzhou 412006) (3.International Engineering Consulting Corporation, Dept. Agriculture, Forestry and Water, Beijing 100044);Study on DNA extraction of Zelkova[J];Bulletin of Botanical Research;2004-01
3 XU Song-jun~1;HSUE Hsiang-hao (XU Xiang-hao)~2 (I. South China Normal University, Guangzhou 510631, China, 2. South China Agricultural University, Guangzhou 510642, China);COMMENTS ON THE TAXONOMIC POSITION OF SOME GENERA IN STERCULIACEAE[J];JOURNAL OF TROPICAL AND SUBTROPICAL BOTANY;2000-01
4 XU Song jun, HSUE Hsiang-hao (XU Xiang-hao) ( Department of Geography, South China Normal University, Guangzhou 510631, China; Biotechnology Collect, South China Agricultural University, Guangzhou 510642, China);GEOGRAPHICAL DISTRIBUTION OF STERCULIACEAE[J];Journal of Tropical and Subtropical Botany;2001-01
5 Niu Xuyan, Wu Wenjun, Liu Huqi and Wu Guomin (Department of Plant Protection, Northwestern Agricultural University, Shaanxi Yangling\ 712100) \ \;Primary Studies on the Fungicidal Activity of Saxifragaceae Plants[J];ACTA AGRICULTURAE BOREALI-OCCIDENTALIS SINICA;1996-02
6 Zhugc Ren (Southwestern Forestry College, Kunming);THE CLADISTIC ANALYSIS OF THE SYSTEMATIC POSITION OF CRAIGIA[J];Acta Botanica Yunnanica;1989-01
7 ZUO Guo-Ying1,2,LI Zheng-Quan1,Chen Li-Rong1,HE Hong-Ping3,XU Xiao-Jie1(1 College of Chemistry and Molecular Engineering,Yangshengtang Nature Medicine Laboratory,Peking University,Beijing 100871,China;2 Kunming General Hospital,Kunming 650032,China;3 The State Key Laboratory of Phytochemistry and Plant Resources in West China,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming 650204,China);Gallic Acid Esters of Bergenin from Saxifraga melanocentra (Saxifragaceae) and Their Inhibition Against HCV NS3 Protease[J];Acta Botanica Yunnanica;2007-04
8 SHI Su-Hua HUANG Ye-Lin ZHANG Qun JIN Hong TAN Feng-Xiao ZHANG Hong-Da (School of Life Sciences, Zhongshan University, Guangzhou 510275);Analysis of the Its Sequences of Nuclear Ribosomal DNA from Tetrathyrium(Hamamelidaceae) and the Related Genera and the Phylogenetic Significance[J];ACTA BOTANICA YUNNANICA;1999-01
9 Wang Xiao-quan Han Ying Deng Zheng-rong Hong De-yuan (Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093);PHYLOGENY OF THE PINACEAE EVIDENCED BY MOLECULAR BIOLOGY[J];Acta Phytotaxonomica Sinica;1997-02
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