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《Journal of Anhui Agricultural Sciences》 2009-01
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Effects of NaCl Stress on Some Physiological Indices of Transgenic Lespedeza formosa with BADH Gene

YANG Xiao-hong et al (Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants,Beijing Forestry University,Beijing 100083)  
[Objective] The aim of this study is to explore the effects of exogenous gene introduction on osmotic adjustment of plants.[Method] After pot culture with the same time,the transgenic plants expressing betaine aldehyde dehydrogenase gene(BADH) and non-transgenic plants of Lespedeza formosa were treated with different concentrations of NaCl(0,0.5%,1.0%,2.0%) for 30 h and some physiological indices including proline,betaine,soluble sugar,malondialdehyde(MDA) contents and SOD activity were assayed.[Result] There was no significant difference on the indices mentioned above between transgenic plants and non-transgenic plants under salt-free treatment.With the increase of NaCl concentration,the transgenic plants accumulated much higher level of proline,betaine,soluble sugar and lower level of MDA than non-transgenic plants.But the differences of SOD activity increase degrees between transgenic and non-transgenic plants were not significant.Performances of the three transgenic lines were different to some extent.[Conclusion] The introduction of exogenous BADH gene enhanced the osmotic adjustment ability of Lespedeza formosa;furthermore,different transgenic lines performed differently.
【Fund】: 国家高技术研究发展计划(2002AA241111);; 北京市教委项目(KM200710020007);; 北京农业应用新技术重点实验室开放课题
【CateGory Index】: S541.5
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