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《Journal of Anhui Agricultural Sciences》 2009-01
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Study on Extraction and Separation of Inulin from Jerusalem artichoke

GAO Jian et al (College of Pharmaceutical Engineering and Life Science,Nanjing University of Technology,Nanjing,Jiangsu 210009)  
[Objective] The aim was to study the extraction and isolation method of inulin from Jerusalem artichoke.[Method] After pretreatment,J.artichoke was grinded to obtain the crude inulin.Through impurity removal by lime milk,protein removal with Sevag method,decoloration with active carbon and ethanol precipitation,the crude inulin was extracted and purified to get the refined inulin.The content of total sugar and reducing sugar was measured.[Result] The decoloration effect of powdery active carbon produced by TS4 manufacturer on the crude inulin extract was the best.After purification,the crude inulin of J.artichoke obtained by hot water extraction method was clear and transparent.After the purification solution was concentrated and precipitated with ethanol,the yellow purified inulin was obtained.Its inulin content was 20.23% and its purity was 92.60%.[Conclusion] The optimum extraction method of inulin from J.artichoke was that hot water extraction method was taken with solid-liquid ratio of 1∶15,extraction for 90 min at 80 ℃,and through the impurity removal by lime milk,protein removal with Sevag method and decoloration with active carbon,the crude inulin could be purified by ethanol precipitation.
【Fund】: 盐城市科技发展计划资助项目(YK2008050)
【CateGory Index】: TQ28
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