Optimization Techniques for the Extraction of Polysaccharides from Mulberry Leaves by Complex Phosphoesterasum
XIA Ping et al (Faulty of Life Science,Huzhou Normal College,Huzhou,Zhejiang 313000)
[Objective] The Complex Phosphoesterasum was used in the experiment to extract polysaccharide from mulberry leaves.[Method] Single factor tests and orthogonal experiment design methods were applied to analyze the influence of the factors such as the concentration,time,temperature and pH value of the enzyme on the extraction ratio of polysaccharides from mulberry leaves.[Results] The adoption of new extraction methods improved the extraction ratio of the target products.[Conclusion] The best conditions are as follows: temperature is 50 ℃,pH value is 4.5,the concentration of cellulose enzyme is 1.0% and the extracting time is 1 h.The extraction ratio of Polysaccharides in mulberry leaves is up to 14.32%.
【CateGory Index】： S888