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Antioxidant Activity Analysis and Identification of an Endophytic Fungal Strain from Eucommia ulmoides Oliv

XIE Hui et al (College of Life Sciences,Nanjing Normal University,Nanjing,Jiangsu 210046)  
[Objective] The aim was to study the antioxidant activity and strain identification of an endophytic fungal from Eucommia ulmoides Oliv.[Method] The tissue separation method was used to screen the endophytic fungi in the leaves of E.ulmoides.Strain No.173 showed highest antioxidant activity and could be used as tested strain.Its broth extract was prepared to determine its antioxidant activity. Strain No.173 was identified from morphology and molecule.[Result] After treatment for 4 d,the POV value of treatment group was significantly lower than that of control group.From 6th d,the effect of treatment group was better than that of positive control group(0.04%Vc and VE).The colony of strain No.173 was white at the primary stage on the PDA plate and gradually changed to dark blue.Its back was brown and the edge of colony was irregular.The result of sequence alignment showed that the similarity between the base sequence of rDNA-ITS amplified fragment of strain No.173 and that of Chaetomium globosum Kunze was 100%.[Conclusion] Endophytic fungi strain No.173 in E.ulmoides leaves belonged to C.globosum and had significant and stable antioxidant activity.
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