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《Journal of Anhui Agricultural Sciences》 2009-02
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Comparision of Peroxidase Isozyme Zymogram and Enzymatic Activity in Several Kinds of Hippophaes rhamnoides L.Leaves

HE Shi-min et al(Department of Life Science,Changjiang Normal College,Fuling,Chongqing 408100)  
[Objective] The objective was to provide the reference basis for the breeding,development and utilization of Hippophaes rhamnoides L.[Method] With the tender leaves of 3 H.rhamnoides varieties(Qiji 1,Alieyi,Chuyi) as materials,their POD zymogram and activity of peroxidase were determined.[Result] The result of staining zymogram showed that the enzyme activity of Qiji 1 was the strongest,the following was that of Alieyi and that of Chuyi was the lowest.POD isozyme zymogram bands of Qiji 1 was the most,being 9 bands with 5 deep color bands.Alieyi had 4 bands with 2 deep color bands.Chuyi had 3 bands with 1 deep color band.The bigger the value of △OD/(min·g)was,the stronger the enzyme activity was.The △OD/(min·g)value of Qiji 1,Alieyi and Chuyi was 0.112,0.100 and 0.092 resp.[Conclusion] The POD activity of 3 kinds of tested H.rhamnoides in order was Qiji 1AlieyiChuyi,and their stress resistance in order was Qiji 1AlieyiChuyi.
【Fund】: 长江师范学院重点扶持学科资助项目
【CateGory Index】: S793.6
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