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《Journal of Anhui Agricultural Sciences》 2009-02
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Study on Regeneration System of Wild Lilium concolor in vitro Culture

YANG Wei-ru et al(College of Landscape Architecture,Beijing Forestry University,National Engineering Research Center for Floriculture,Beijing 100083)  
[Objective] The aim was to explore the suitable tissue culture method for Lilium concolor.[Method] With different parts of squama from wild L.concolor as the explant,the effects of different sterilizing times and mediums on the explant tissue culture process were studied.[Result] The effects of different sterilizing times on the contamination rate of the squama were different and they all showed downtrend with the prolonging of the sterilizing time.The contamination rate of the squama among different parts were significantly different,and among them,the contamination rate of the external squama was highest,followed by the middle part and that of the internal squama was lowest.The multiplication coefficient of the different parts of squama showed gradual downtrend with the increasing of the sterilizing time by the mercuric chloride.With the middle squama as the explant,the effect of the sterilizing time of 6+6(min) was better,then the contamination rate was 59.5% and the multiplication coefficient was 2.57.The suitable medium for inducing the adventitious bud was MS+NAA 0.1 mg/L+6-BA 2.0 mg/L and the multiplication coefficient was 4.08.[Conclusion] The research laid the foundation for protecting the L.concolor germplasm resources and developing the variety with the independent intellectual property.
【Fund】: “十一五”科技支撑计划课题(2006BAD01A08)
【CateGory Index】: S682.29
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