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Study on Antibacterial Activity of Rubia cordifolia L.on the Major Pathogenic Bacteria Causing Mastitis

WANG Zuo-xin et al(Department of Veterinary Medicine of Gansu Agricultural University,Lanzhou,Gansu 730070)  
[Objective] To study antibacterial activity of different purification of Rubia cordifolia L.ethanol extracts.[Method] Selecting Staphylococcus aureus,Streptococcus dysgalactiae,Streptococcus agalactiae Bacillus coli,Bacillus proteus,Bacillus aeruginosus as representative strain,fractional extraction was conducted to Rubia cordifolia L.root ethanol extracts by solvents with different polarities to screen the partial purification from main pathogen which had inhibitory effect on the mastitis of cows.[Result] Petroleum ether,chloroform ether and ethyl acetate extracts from Rubia cordifolia L.has inhibitory effect,among which the inhibitory activity of ethyl acetate soluble fractions of the plant was most significant to the 5 germs;petroleum ether and chloroform ether soluble fraction of the plant had similar antibacterial activity to 6 germs;n-butyl alcohol is insensitive to the 6 germs and acetone is the most inactive.
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