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《Journal of Anhui Agricultural Sciences》 2009-02
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Content Determination of Polysaccharide in Aconitum carmichaili of Jiangyou

ZHAO Xiang-sheng et al(College of Life Science and Engineering,Southwest University of Science and Technology,Mianyang,Sichuan 621010)  
[Objective] To determine the polysaccharide content in Aconitum carmichaili.[Method] The polysaccharide in Aconitum carmichaili was filtrated by hot water after back-flowing with 80% ethanol and assayed by Anthrone-sulfuric acid colorimetry.Glucose was used as standard in the preparation of calibration curve.[Result] The results proved that the method was simple and the color of the treated solution showed quite good stabilization within 6h and the average value of the recovery for polysaccharide measurement was 90.4% with 1.58% of RSD(n=5).The contents of polysaccharide in Aconitum carmichaili of Jiangyou was 3.34% with 2.31% of RSD.[Conclusion] Anthrone-sulfuric acid could be used as the optimal way to detect polysaccharide content in Aconitum carmichaili.
【Fund】: 国家“十一五”科技支撑计划重大专项课题(2007BAD89B15)
【CateGory Index】: R284
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