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《Journal of Anhui Agricultural Sciences》 2009-03
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Effects of Cultural Conditions on Flavonoids Formation in Different Explant Callus of Glycyrrhiza uralensis Fisch

CAO Jun-mai et al(College of Life Sciences and Engineering,North Nationality University,Yinchuan,Ningxia 750021)  
[Objective] The research aimed to screen the best explant,medium formula and cultural conditions for producing flavonoids of secondary metabolites.[Method] Under different cultural conditions,the effect of 5 exogenous hormones of ZT,ABA,NAA,KT and 6-BA on formation of flavonoids in callus induced by roots and buds of Glycyrrhiza uralensis Fisch was studied by using the methods of orthogonal experiment design of L16(45) and contrast experiment design.[Result] The T-test indicated that weak light(100-300 lx) was beneficial to forming of flavonoids production;the callus induced from root was a suitable explant for forming of flavonoids yield.F-test showed that 5 exogenous hormones had extremely significant difference on forming of flavonoids production,the effect on total flavonoids content in the callus induced by root was in the such order as: KT6-BAZTABANAA;the effect on total flavonoids content in the callus induced by bud was in the such sequence as: 6-BAKTNAAABAZT.The optimal hormone combinations obtained after optimization were: ABA 1.0 mg/L + KT 2.0 mg/L +ZT 1.0 mg/L +6-BA1.0 mg/L + NAA 2.0 mg/L.[Conclusion] The study provided a theoretical basis for industrialized production of secondary metabolites of flavonoids.
【Fund】: 宁夏基金项目(Nz0688)资助
【CateGory Index】: S567.71
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