Screening on Multiplication Medium of Tissue Culture for Anoectochus roxburghih
LIU Wei et al(Biochemistry Department,Wenshan Teachers College,Wenshan,Yunnan 663000)
[Objective] The study aimed to screen the optimum multiplication medium of tissue culture for Anoectochus roxburghih.[Method] The stem segment and terminal bud of the tissue culture seedlings of A.roxburghih were taken as the explant for multiplication culture to compare the effects of factors such as different basic culture medium,cytokinin,growth hormone,supplement matter on the growth of A.roxburghih under the culture conditions as light time of 12 h/d,light intensity of 1 200 lx and temperature of(25±2) ℃ and pH of 5.8.[Result] After the comparative analysis on the culture effect of different media,the screened optimum media were as follows: the optimum formula for budding multiplication was MS + agar 7 g/L + sucrose 25 g/L + NAA 0.2 mg/L + 6-BA 2 mg/L;the strong sprout media were MS+ agar 7 g/L+ sucrose 25 g/L + protein peptone 2 g/L+ Huabao No.1 3 g/L+ banana 100 g/L+ activated charcoal 1 g/L and 1/2MS + agar 7 g/L+ sucrose 25 g/L + protein peptone 2 g/L + banana 100 g/L+ activated charcoal 1 g/L.[Conclusion] The supplement matter such as potato,banana,protein peptone,Huabao No.1 and activated charcoal could improve the differentiation and growth of A.roxburghih explant.