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《Journal of Anhui Agricultural Sciences》 2009-04
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The Antigen Analysis of Eperythrozoon canis

QI Kai et al (Veterinary Department,Agricultural College,Yanbian University,Longjing,Jilin 133400)  
[Objective] This study aimed to grasp antigen traits of Eperythrozoon canis.[Method] After culturing Eperythrozoon canis in vitro and splitting the dissociated Eperythrozoon canis with ultrasonic wave,this study analyzed the extracted antigen by SDS-PAGE and immunoblotting.[Result] 3 main protein belts appeared on the electrophoretogram of Eperythrozoon canis,their molecular weight were respectively 66,32 and 25 KD,among which antigen of 32 and 25 KD had better histocompatibility and would not cross react with negative serum of Eperythrozoon canis.[Conclusion] This research provided foundations for the antigen of Eperythrozoon canis and immunological diagnosis of Eperythrozoon canis diseases.
【CateGory Index】: S858.292
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