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《Journal of Anhui Agricultural Sciences》 2009-04
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Rapid Propagation and Plant Regeneration of Erigeron breviscapus(Vaniot) Hand.-Mazz.

LIN Li-fei et al (Department of Biology,Honghe University,Mengzi,Yunnan 661100)  
[Objective] To establish a systematic mechanism of Erigeron breviscapus tissue culture for culturing,strengthening and developing an advantageous industry with unique characteristics.[Method] The leaves of Erigeron breviscapus were used as explants to induce callus,buds and become to plant regeneration in different optimal medium.[Result] MS+KT 0.5 mg/L+2,4-D 10 mg/L and MS + 2,4-D 0.5 mg/L+ 6-BA 0.5 mg/L were best to induce callus,comparatively,the latter was looser than the former.The optimal medium for bud differentiation was MS+6-BA 0.5 mg/L +10% banana and differentiation rate was 87%.And that 1/2MS with 0.3 mg/L NAA was suitable for rooting and the rooting rate was 83%.[Conclusion] Rational ratio of auxin and cytokinin was favorable for quickly establishing a systematic mechanism of Erigeron breviscapus culture.
【Fund】: 云南省自然科学基金(2003C0044M);; 红河学院硕士专项课题(XSZ05030)
【CateGory Index】: S567.239
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