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《Journal of Anhui Agricultural Sciences》 2009-04
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Studies on the Culture Condition and Fermentation Crafts of A Strain of Bacillus subtilis

LI Xiao-Jun et al(Life Science College,Hebei Normal University,Shijiazhuang,Hebei 050016)  
[Objective]The purpose was to optimize the culture conditions and fermentation crafts of strain A,a kind of Bacillus subtilis(CGMCC No.1982),and in order to obtain high spores concentration of fermentation broth and establish scientific foundation for empoldering the strain A.[Method] Uniform design tests,regression analysis method and fed-batch fermentation techniques were used.[Result] The results showed that the optimum conditions for shaking flask culture of strain A were as follows: 35 ℃ of culture temperature,culture broth quantity 12%,inoculation amount 3.0%,initial pH value 7.2.The fed-batch fermentation crafts were ascertained by 20 L fermenter.The seed was prepared by using YDC as seed culture medium(glucose 20 g,yeast extracts 10 g,CaCO3 20 g and water 1 000 ml),culture time 24 h and inoculation amount 3.0%.The fermentation medium(glucose 1.0%,yeast extracts 1.0%,CaCO31.0% and pH value 7.2) and the feeding medium,a mixture of glucose 1.5% and yeast extracts 0.5%,were confirmed.The feeding began at 8 h later and the fermenting period lasted for(34±2) h,the spore concentration could reach to(3.410±0.151) ×109 cfu/ml.[Conclusion]The culture conditions and fermentation crafts of strain A to produce high concentration of spores was validated,which will establish scientific foundation for empoldering the strain A.
【Fund】: 河北省科技攻关计划(05547002D-5);; 石家庄市科技攻关计划(07149113A);; 河北师范大学应用开发基金(L2004K02)资助项目
【CateGory Index】: S476.1
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2 LI Xiao-jun,CHENG Li-xia,WU Yan-bin,BIAN Yan-qing(College of Life Science,Hebei Normal University,Shijiazhuang 050016,China);New Methods of Testing Fermentation Liquid Antagonistic Effect of Antagonistic Bacteria and Antimicrobial Spectrum[J];Biotechnology;2007-01
3 LI Jin-yun, WANG Hui-min, WANG Jian-hui (Department of Plant Pathology, China Agricultural University, Beijing 100094);A Bacteriocin with a Broad Spectrum Activity Produced by Grapevine Crown Gall Biocontrol Strain E26[J];Scientia Agricultura Sinica;2004-12
4 Zhang Xuejun ling Hongtong Li Honglian Wang Jinsheng (Dept. of Plant Protection, Nanjing Agricultural University, Nanjing 210014);INHIBITION OF MAIFENGNING B_3(BACILLUS SP. ,A BIOLOGICAL FUNGICIDE) TO WHEAT SHARP EYESPOT (RHIZOCTONIA CEREALIS)[J];Acta Phytopathologica Sinica;1994-04
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【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Zhang Shumei,Wang Yuxia,Wang Jialong,Li Jing(Institute of Applied Microbiology of Heilongjiang Academy of Sciences,Harbin, 150010);Biocontrol of soybean Sclerotinia sclerotiorum by Bacillus subtilis[J];Soybean Bulletin;2006-01
2 Liang Zhihong Wang Huimin Wang Jianhui(College of Plant Protection, CAU);Preliminary Study on Effectiveness and the Stability of E26 on Controlling Crown Gall Disease[J];Journal of China Agricultural University;2001-01
3 SONG Xiao-yan,CHEN Xiu-lan,SUN Cai-yun,XIE Shu-tao & ZHANG Yu-zhong~*(State Key Laboratory of Microbiol.Technology,Shandong Univ.,Jinan 250100,Shandong,China);Screening of antagonistic Trichoderma spp.against Verticillium dahliae and study on its mechanism of action[J];Journal of Shandong University(Natural Science);2005-06
4 Tian Yu Hong Fang Hu Cheng Wang Zhongyan (College of Life Science,Sichuan University,Chengdu 610064);Selection of an Bacteriocin-like Substance Producing Strain and Study on Its Fermentation Conditions[J];Food and Fermentation Industries;2004-03
5 LIU Jing WANG Jun YAO Jian Ming PAN Ren Rui YU Zeng Liang * (Key Laboratory of Ion Beam Bioengineering,Chinese Academy of Sciences, Hefei 230031, China);Properties of The Crude Extract of Bacillus subtilis and Purification of Antimicrobial Peptides[J];Acta Microbiologica Sinica;2004-04
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7 WANG Hui-min,SUN Yan-li, WANG Jian-hui(College of Plant Protection , China Agricultural University, Beijing 100094);Preliminary Study on the Effects of Biocontrol Strain E26 on Some Ecological Factors[J];Scientia Agricultura Sinica;2002-01
8 LIU Hai bo, TIAN Shi ping, QIN Guo zheng, XU Yong (Institute of Botany, Chinese Academy of Sciences, Beijing 100093);Effect of Cryptococcus laurentii on Biological Control of Diseases in Postharvest Grapes[J];Scientia Agricultura Sinica;2002-07
9 HE Hong, CAI Xueqing, HONG Yongcong, GUAN Xiong, HU Fangping(College of Plant Protection, Fujian Agri. and Forestry Univ., Fuzhou 350002, China);Selection of Endophytic Antifungal Bacteria from Capsicum[J];Chinese Journal of Biological Control;2002-04
10 FENG Shuliang, WANG Rongyan, LIN Kaichun, ZHANG Yongmei, DU Lixin, FAN Xiuhua, CAO Weiping (Institute of Plant Protection, Hebei Academy of Agriculture & Forest Science, Baoding 071000, China);Identification of Strain Bs-208 and Its Inhibition Against Plant Pathogenic Fungi[J];Chinese Journal of Biological Control;2003-04
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