Studies on the Culture Condition and Fermentation Crafts of A Strain of Bacillus subtilis

LI Xiao-Jun et al(Life Science College,Hebei Normal University,Shijiazhuang,Hebei 050016)  

[Objective]The purpose was to optimize the culture conditions and fermentation crafts of strain A,a kind of Bacillus subtilis(CGMCC No.1982),and in order to obtain high spores concentration of fermentation broth and establish scientific foundation for empoldering the strain A.[Method] Uniform design tests,regression analysis method and fed-batch fermentation techniques were used.[Result] The results showed that the optimum conditions for shaking flask culture of strain A were as follows: 35 ℃ of culture temperature,culture broth quantity 12%,inoculation amount 3.0%,initial pH value 7.2.The fed-batch fermentation crafts were ascertained by 20 L fermenter.The seed was prepared by using YDC as seed culture medium(glucose 20 g,yeast extracts 10 g,CaCO3 20 g and water 1 000 ml),culture time 24 h and inoculation amount 3.0%.The fermentation medium(glucose 1.0%,yeast extracts 1.0%,CaCO31.0% and pH value 7.2) and the feeding medium,a mixture of glucose 1.5% and yeast extracts 0.5%,were confirmed.The feeding began at 8 h later and the fermenting period lasted for(34±2) h,the spore concentration could reach to(3.410±0.151) ×109 cfu/ml.[Conclusion]The culture conditions and fermentation crafts of strain A to produce high concentration of spores was validated,which will establish scientific foundation for empoldering the strain A.

【Fund】： 河北省科技攻关计划(05547002D-5);; 石家庄市科技攻关计划(07149113A);; 河北师范大学应用开发基金(L2004K02)资助项目
【CateGory Index】： S476.1

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