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《Journal of Anhui Agricultural Sciences》 2009-04
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Determination of Three Medicinal Bioactive Components of Swertia franchetiana H.Smith

MA Yu-hua et al(Department of Chemistry,Qinghai Nationalities University,Xining,Qinghai 810007)  
[Objective] The purpose was to determine three medicinal bioactive components in different parts of Swertia franchetiana H.Smith.[Method]The content of swertiamarin,gentiopicroside and mangiferin in Swertia franchetiana H.Smith were determined by reversed-phase high performance liquid chromatography.[Result]The optimum conditions of determining three medicinal bioactive components in different parts of Swertia franchetiana H.Smith were as follow: a Kromasil C18(250 mm×4.60 mm,5 μm);the mobile phase was composed of CH3OH and 0.02% phosphoric acid in water;the gradient condition was 0-25 min,CH3OH 20%-50%;the flow rate was 1 ml/min;the detection wavelength was 260 nm.The good linear relationships and recoveries were obtained under the optimum conditions.[Conclusion]The content of gentiopicroside is most high in the root,swertiamarin and mangiferin have higher content in the floral part of Swertia franchetiana H.Smith.
【Fund】: 国家中西部重点项目(2001BA901A47)
【CateGory Index】: R284
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