Scanning Electron Microscope Observation on Cylicostephanus longibursatus
GUO Xiao-lin et al (College of Life Sciences,Hebei Normal University,Shijiazhuang,Hebei 050016)
[Objective] The aim was to observe the head and tail structures of Cylicostephanus longibursatus by scanning electron microscopy.[Method] With the cecum of donkey from Henan Province as tested material,the collected nematodes were fixed in 70% alcohol to observe and indentify and the identified C.longibursatus were observed by scanning electron microscopey.[Result] The head of the C.longibursatus body had developed mouth collar,and 4 submedian papillae extended from the base of the mouth collar to its top and showed uplift and the part outside the mouth collar showed conical.The amphid was ovoid.There was an external leaf crownd around the aperture.The element of leaf crown was narrow and leafy and its tip was slightly round.The tail of male had developed copulatory bursa and a pair of spicules which were slender and linear and its tip had crotchet.The tail of female was conical and its tip was tapered.The anus had transverse crack and was crescent.The vulva was ovoid and there had cuticular plates to support in its foreside and both sides of the inner face,but there was no support in inner surface of the posterior.[Conclusion] The research provided the more detailed basis for the classification and identification of C.longibursatus.