Study on Tissue Culture and Effective Clone Establishment of Hemistepta lyrata Bunge
ZHANG Wen et al(College of Life Science,Liaoning Normal University,Dalian,Liaoning 116029)
[Objective] The research aimed to study the rapid propagation technology and establish effective clone of Hemistepta lyrata Bunge.[Method] With tender stem of Hemistepta lyrata Bunge as material,the conditions needed in callus induction and differentiation,adventitious bud differentiation and radication,test tube seedling cutting and transplantation were studied.[Result] The results showed that the optimum medium for granulated callus induction from tender stem was MS+6-BA 0.3 mg/L+2,4-D 1.0-1.5 mg/L,for granulated callus and adventitious bud differentiation was MS+AgNO3 1.5 mg/L +6-BA 0.4 mg/L +NAA 0.1 mg/L.1/2 MS+IAA 0.6 mg/L was suitable for test tube seedling rooting and regeneration,and cinder was used as transplation and cutting substrate.[Conclusion] This study will provide the scientific reference for choosing the feasible medium in tissue culture of Hemistepta lyrata Bunge.
【CateGory Index】： S567.239