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《Journal of Anhui Agricultural Sciences》 2009-07
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Analysis on Mitochondrial cob Gene Sequences of Otostigmus(O.) politus

ZUO BAO-ping et al(Jiangsu Key Lab for Biodiversity and Biotechnology,College of Life Science,Nanjing Normal University,Nanjing,Jiangsu 210046)  
[Objective]The research aimed to preliminarily discuss the geographic variation of Otostigmus(O.) politus.[Method]The samples of Otostigmus(O.) politus were preserved by 95% ethanol,and then been extracted for its total genome to undergo PCR amplification and sequencing.At last,its sequences were analysized with Clustal X1.83 software.[Result]A total of 26 haplotypes were defined by sequences variance,and 58 individuals were collected from 11 locations in five populations.Phylogenetic tree(Baysian) of haplotypes was very confusion,which showed that the range of genetic diversty was very large,influenced by glacial periods in the Pleistocene.[Conclusion]According to molecular clock of arthropods,the data suggested that the distribution pattern of Otostigmus(O.) politus was very original,which formed after the middle of Tertiary Period.So it wasn′t influenced by glacial periods in the Pleistocene.
【Fund】: 国家自然科学基金重点资助项目(30130040)
【CateGory Index】: Q953
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