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《Journal of Anhui Agricultural Sciences》 2009-07
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Study on the Removal of Protein from Polysaccharides of Momordica charantia L.

GUO Yu-dong et al(Department of Agricultural Science of Yingdong Biological Sciences College,Shaoguan College,Shaoguan,Guangdong 512005)  
[Objective] The research aimed to seek the suitable deproteinization methods from Momordica charantia L.polysaccharides.[Method] The deproteinization effects of Seveage method,enzyme method,enzyme-Seveage method,trichloroacetic acid method and hydrochloric acid method on M.charantia polysaccharides were compared.[Result] The deproteinization effect of enzyme-Seveage method was the best,with the papain dose of 1.25%(V/V),pH 6.5 at 60 ℃ for 2 h.Then the polysaccharide solution was deproteinized again with Seveage agent and the removal rate of protein reached 77.2% and the loss rate of polysaccharide was 19.3%.[Conclusion] The enzyme-Seveage method was an effective deproteinization method,with higher removal of dissociated protein and lower loss of polysaccharide.
【CateGory Index】: TQ914.1
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