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《Journal of Anhui Agricultural Sciences》 2009-08
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Identification of True or False and Purity of Hybrid Rice with SSR Molecular Marker

HUANG Cheng-zhi et al(Three Gorges Academy of Agricultural Sciences in Chongqing,Chongqing 404001)  
[Objective] The aim was to search for the quick and effective method for the identification of true or false and purity of hybrid rice seeds so as to improve the identification efficiency.[Method] The true or false and purities of seeds of the hybrid rice Zhongyou 88,Xieyou 88 and Quanfengyou 88 were identified with SSR molecular marker and the identification result was compared with the field identification result.[Result] Two complementary bands of the parents appeared in the DNA band spectra of the hybrid seeds,which showed that the hybrid seeds were true.The purities of the Zhongyou 88 identified by SSR molecular marker and in field were 97.43% and 97.60%,resp.,that of Xieyou 88 were 98.00% and 98.10%,resp.and that of the Quanfengyou 88 were 96.29% and 96.60%,resp.The results identified by SSR molecular marker and in field were identical basically and had no obvious difference,which showed that using SSR to detect the purity of the hybrid seeds was completely feasible.[Conclusion] The SSR molecular marker had better application value and foreground in the identification of true or false and purity of hybrid rice seeds.
【CateGory Index】: S511
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