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《Journal of Anhui Agricultural Sciences》 2009-08
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Research on the Optimization of SSR-PCR System in Rice

ZHU Shi-yang et al(Department of Agriculture and Biotechnology,Wenzhou Vocational College of Science and Technology;Zhenan Key Laboratory of Crop Breeding,Wenzhou,Zhejiang 325006)  
[Objective] The SSR-PCR system of rice(Oryza sativa L.) was explored.[Method] The rice variety R117 being taken as material,the effect of Mg2+,dNTP,primer,Taq NDA polymerase and temperature on SSR-PCR amplification reaction was studied.[Results] The result showed that the optimal SSR-PCR reaction system in rice included Mg2+,2.00 mmol/L;dNTP,0.15mmol/L;primer,0.30 μmol/L;Taq DNA polymerase,2.0 U and annealing temperature,56.6 ℃,which resulted in the best PCR amplification band.[Conclusion] In the reaction system,the condition would produce the best result.
【Fund】: 温州市科技计划项目(N2006A001)
【CateGory Index】: S511
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