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《Journal of Anhui Agricultural Sciences》 2009-08
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Study on Detection of Polymorphism of No.12 Exon in MxA Gene of Horse

JU Lin-hua et al(College of Animal Science and Veterinary Medicine of Inner Mongolia Agricultural University,Hohhot,Inner Mongolia 010018)  
[Objective] The experiment aimed to establish a fast and accurate method to detect the polymorphism of No.12 exon in MxA gene of horse.[Method] The mismatch PCR was used to amplify the No.12 exon,then the PCR product was conducted RFLP.It was concluded from the identification that the nucleotide 1 790 site in cDNA of MxA gene was mutated and the PCR product was undergone nucleotide sequence analysis.[Result] There were 3 genotypes(AA,AB,BB) in the region of No.12 exon in MxA gene of horse.The bases of nucleotide 1 790 site in cDNA of MxA gene were mutated(G was changed to C) and this mutation caused the change of tryptophan to cysteine.The specific sequence of PCR-RFLP product deserved from mismatch PCR was same to that deserved from RFLP analysis.[Conclusion] The mismatch PCR-RFLP method was easy to detect No.12 exon in MxA gene of horse and the result was accurate.
【Fund】: 内蒙古自治区自然基金资助项目(200508010413)
【CateGory Index】: S821
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