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《Journal of Anhui Agricultural Sciences》 2009-08
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Determinati on of Verbascoside in Pedicularis davidii by HPLC

MA Fu-jun(Department of Chemistry,Teachers College for Nationalities,Qinghai Normal University,Xining,Qinghai 810008)  
[Objective]To establish an analytical method for the determination of verbascoside in Pedicularis davidii by HPLC.[Method]By using HPLC,the conditions of chromatography: Kromasil C18(250 mm × 4.6 mm,5 μm) was used with a mobile phase of CH3CN-1%CH3COOH(1∶4),to determine verbascoside content in the sample with wavelength 330 nm.[Result] With high specificity,the average spiking recovery rate was 96.87%,RSD value was 2.18%(n=5).[Conclusion]This method was convenient and had an ideal result in the separation,which could be adopted in the identification and quality control of Pedicularis davidii.
【CateGory Index】: S567.239
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