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《Journal of Anhui Agricultural Sciences》 2009-10
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Application of BA and NAA in the Rapid Propagation in vitro of Tamarix chinensis

LI Xian-fang et al(Department of Medicine Engineering,Zhengzhou College of Animal Husbandry Engineering,Zhengzhou,Henan 450011)  
[Objective] The purpose was to study the influences of BA and NAA on the induction and multiplication of Tamarix chinensis multiple bud clumps and the formation of its adventitious roots.[Method] The T.chinensis branches were cut into 1.5-2.0 cm segments and incubated on the induction medium MS+BA 0-6.00 mg/L+NAA 0-0.50 mg/L for adventitious bud induction.[Result] When the concn.of BA and NAA were 0,99% explants generated 1-2 white roots with length of 1-5 cm on the basal eustipes and their lateral roots were sparse,but there was no multiple bud clumps generated and their branches and leaves grew badly.When the concn.of BA and NAA were 2.00 and 0 mg/L resp.,it was most suitable for adventitious bud induction.When the concn.of NAA was constant,as the concn.of BA was increased,the generation of T.chinensis multiple bud clumps was advanced and the multiplicative coefficient was also increased gradually,but the differentiation of stem was inhibited gradually.MS+BA 0.50 mg/L +NAA 0.01 mg/L was suitable for the multiplication of multiple bud clumps.When the concn.of NAA was 0.50 and 1.00 mg/L,the generation of main and lateral roots was not obvious and there was callus generated at the base of rootless seedling.When MS medium was used in the rooting culture of T.chinensis,the suitable addition concn.of NAA was 0.05 mg/L.[Conclusion] The study provided experimental foundation for the tissue culture of T.chinensis.
【Fund】: 河南省科技厅科技攻关计划项目(0424050018)
【CateGory Index】: S793.5
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