Establishemnt of SSR Fingerprint Map and Analysis of Genetic Similarity among 42 Parents of Japonica Hybrid Rice
LIU Hai-zhen et al(Agricultural College,Anhui Agricultural University,Hefei,Anhui 230036)
[Objective] The study aims to research SSR method establishing the SSR fingerprinting map of parents of japonica hybrid rice.[Method] The genomic DNA of 42 parents of japonica hybird rice is used for PCR analysis with 48 pairs of SSR primers,of which 12 primers are selected as core primers because of their stable polymorphism.A database of SSR fingerprint for 42 genotypes at the 12 primer loci is constructed.[Result] The genetic similarity of 42 genotypes is calculated by cluster analysis of an un-weighted pair-group average method with arithmetic mean,the result shows that 42 varieties of rice can be classified into 5 groups at the level of genetic similarity coefficient is 0.73.[Conclusion]The result of cluster basically reflects the genetic relationships among varieties.