Protective Effects of NO and H_2O_2 on Seed Germination and Seedling Growth of Horsebean under Cd Stress
LI Yuan et al(College of Life Science and Technology,Gansu Agricultural University,Lanzhou,Gansu 730070)
[Objective] The aim was to discuss the physiological and biochemical mechanism of NO and H2O2 on horsebean under Cd stress.[Method] The solution culture was used to research the effect of soaking treatment with sodium nitopresside(SNP,as NO donor),hydrogen peroxide(H2O2) and their combination(with volume ratio of 1∶1) on seed vigor of horsebean under Cd stress and the soil culture was used to research the protective effects of above 3 treatments on oxidative damage of horsebean seedlings under Cd stress.[Result] Compared with single treatment of SNP and H2O2,their combined interaction treatment could increase the seed vigor of horsebean under Cd stress remarkably so as to relieve the toxicity effect of heavy metal Cd on seed germination of horsebean and the interaction treatment of SNP and H2O2 had positive cooperative effect on relieving the oxidative damage of horsebean seedlings under Cd stress,which showed to enhance the vigor of root,increase the chlorophyll content and proline content and decrease the MDA content(P0.05).[Conclusion] The interaction treatment of NO and H2O2 could weaken the degradation reaction of chlorophyll effectively and enhance the osmotic adjustment ability and toxic tolerant ability of leaf cells.