Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui Agricultural Sciences》 2009-13
Add to Favorite Get Latest Update

Study on the Optimization of the Technological Conditions of Walnut Protein Hydrolyzeed by Papain and the Antioxidative Activity of Its Hydrolysate

LIU Zhao-ming et al (Department of Biological and Chemical Engineering,Guangxi University of Technology,Liuzhou,Guangxi 545006)  
[Objective] The research aimed to investigate the optimum technological conditions of walnut protein hydrolyzed by papain and antioxidative activity of its hydrolysate.[Method] The optimum technological conditions of walnut protein hydrolyzed by papain were confirmed by orthogonal experiment.The enzyme hydrolysate was separated by Sephadex G-25 gel filtration and the antioxidative activities of hydrolysate were studied through determining the scavenging activities of hydroxyl and superoxide anion free radicals.[Result] The optimum conditions for the papain hydrolysis of walnut protein were as follows: the temperature of 55 ℃,substrate concentration of 5%,enzyme concentration of 60 000 U/g and pH of 8.0.When the hydrolysis degree was 38.4%,the scavenging rate of its enzymolysis products to hydroxyl free radicals superoxide anion free radicals were 22.5% and 46.4% respectively.Four fractional peptides were obtained from hydrolysate by Sephadex G-25 gel filtration.Among them,the scavenging capability of the peptide C was the biggest.[Conclusion] The papain hydrolysate of walnut protein had certain antioxidative activity.
【Fund】: 广西区教育厅科研立项项目(200708LX184);; 广西工学院食品科学硕士学位授权点学科建设经费资助项目(院科自0705104)
【CateGory Index】: TS201.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 Lü Mingrui1,SHI Xuanming1,ZHANG Li1,MENG Jia1, WANG Ning1,WANG Fengtao1,LIAO Xingmao2(1.Xi'an Oils & Fats Research & Design Institute,State Administration of Grain Reserve of P.R.C., Xi'an 710082,China;2.Gardening,Sillworm and Mulberry Station of Agrotechnical Center in Hanzhong,Hanzhong 723000,Shaanxi,China);Progress on functional properties and preparation of walnut polypeptides[J];China Oils and Fats;2013-05
2 ZHAI Meng-xin,CUI Li,GUO Miao,ZHANG Shi-xin,WANG Tao,XU Mei-yu(Department of Food Science and Engineering,College of Biological Sciences and Technology/ Beijing Forestry University Beijing Key Laboratory of Forest Food Processing and Safety,Beijing 100083,China);Research Progress on Bioactivity of Walnut Protein[J];Food and Nutrition in China;2013-06
【Citations】
Chinese Journal Full-text Database 4 Hits
1 LIU Zhao-ming et al(Department of Biological and Chemical Engineering,Guangxi University of Technology,Liuzhou,Guangxi 545006);Study on Preparation and Antioxidant Activity of Hydrolysate from Neutral Protease in Walnut Protein[J];Journal of Anhui Agricultural Sciences;2008-35
2 ;核桃蛋白酶解产物抗氧化性的研究[J];Science and Technology of Food Industry;2007-04
3 XU Ying-cao, LIU Chun-hong (Department of Chemistry ,College of Science , Northeast Agriculture University,Harbin 150030,Heilongjiang, China);INTRODUCTION OF METHOD FOR DETERMINATION OF DEGREE OF HYDROLYSIS OF PROTEIN HYDROLYSATES[J];Food Research and Development;2007-07
4 SHI Jun,WANG Jin-shui,CAI Feng-ying,LIU Jin-xi(School of Food Science and Technology,Henan University of Technology,Zhengzhou 450052,China);STUDY ON ENZYMATIC HYDROLYSIS OF PEANUT PROTEIN AND ANTIOXYGENIC PROPERTY OF ACTIVE PEPTIDES[J];Journal of Henan University of Technology(Natural Science Edition);2006-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Chen et al(College of Life Science,Yangtze University,Jingzhou,Hubei 434025);Study on Antioxidant Activity of Extracts of Celery Leaf[J];Journal of Anhui Agricultural Sciences;2006-13
2 ZHANG Ji et al(College of Life Sciences,Northwest Normal University,Lanzhou,Gansu 730070);Research on the Scavenging Capability of Free Radical[J];Journal of Anhui Agricultural Sciences;2008-09
3 LIU Zhao-ming et al(Department of Biological and Chemical Engineering,Guangxi University of Technology,Liuzhou,Guangxi 545006);Study on Preparation and Antioxidant Activity of Hydrolysate from Neutral Protease in Walnut Protein[J];Journal of Anhui Agricultural Sciences;2008-35
4 LIU Ling et al(Institute of Bioresource R&D of Kunming University of Science and Technology-Tsinghua University,Kunming,Yunnan 650224);Recent Progress on the Function of Walnut[J];Journal of Anhui Agricultural Sciences;2009-27
5 CHEN Xiao-ming et al(College of Life Science and Chemical Engineering,Huaiyin Institute of Technology,Huaian,Jiangsu 223001);Research on the Preparation Technology of the Antioxidant Peptide from the Waste of Freshwater Procambarus clarkia with Enzymatic Hydrolysis[J];Journal of Anhui Agricultural Sciences;2011-22
6 HUANG Liang et al(Department of Agriculture,Tianjin Agricultural University,Tianjin,300384);Study on the Antimicrobial Activity of Ethanol Extracts from H.cordata Leaves[J];Journal of Anhui Agricultural Sciences;2012-02
7 HE Yue-jun,YUE Yong-de,TANG Feng(International Center for Bamboo and Rattan,Beijing 100102);Detection of antioxidative capacity of essential oils from the bamboo leaves by scavenging organic free radical DPPH[J];Journal of Anhui Agricultural University;2009-03
8 LIU Shao-jun1,NIU Ying2,LIU Bing-hao2,CHEN Guo-ping2(1.College of Life Science,Guangxi Normal University,Guilin,Guangxi 541004;2.Guangxi Citrus Research Institute,Guilin,Guangxi 541004);Study on Determination of the Content of Reduction-type VC in Chinese Grapefruit's Sarcocarp with Phosphomolybdate-blue Spectrophotometry[J];Northern Horticulture;2011-01
9 JIANG Li1,XU Wen-bo1,WANG Yu-rong1,ZHAO Shao-hua2 (1Beijing University of Chinese Medicine,Beijing 100102,China;2Hebei Yiling Pharmaceutical Group,Shijiazhuang 050035,China);Kinetics and its influencing factors of enzymatic process on scorpion[J];China Journal of Traditional Chinese Medicine and Pharmacy;2010-12
10 LUO Yang-he1,FAN Yu1,JIANG Zhi-liang2,XIE Qing-lin1(1.Department of Chemical and Biological Engineering,Hezhou University,Hezhou Guanxi 542800,China;2.Department of Material and Chemical Engineering,Guilin University of Technology,Guilin 541004,China);Determination of Antioxidant Activity of Eleochairis Toberosa Peel by Spectrophotometry with Acridine Red[J];Journal of Jiangxi Normal University(Natural Sciences Edition);2008-03
China Proceedings of conference Full-text Database 5 Hits
1 Chen Qinghua~1,~*Wang Jinshui~2,Li Lu~2,Wei Zhiyan~2,Li Jun~2 (1.Yongcheng Vocational College,Yongcheng,He'nan 476600,China; 2.College of Grain Oil and Food Science,He'nan University of Technology,Zhengzhou,He'nan 450052,China);Stability of Antioxygenic Property of Peanut Protein Bioactive Peptides[A];Chinese Society of Agricultural Engineering[C];2009
2 Chen Zhiyi,Wu Jijun,Liu Xueming,Li Shengfeng (The Sericulture & Farm Produce Processing Research Institute of Guangdong Academy of Agricultural Sciences,Guangdong Open Access Laboratory of Agricultural Product Processing,Guangzhou 510610, China);Study on Aryltetranap lignanoids and it's antioxidant effect in plum wine[A];[C];2008
3 ;The study on enzymatic process of scorpion and preliminary identification of the hydrolysates[A];[C];2009
4 LI Yuding 1, 2 , YE Xiaohui 3 , YE Naixing 1, 3 , LIN Zhida 3 , ZHENG Deyong 1 , CHEN Xinghuang 2 (1. Institute of ScienceTechnology and Economics of Tea Industry,Fuzhou Fujian, 350002, China ; 2. College of Food Science,Fujian Agriculture and Forestry University,Fuzhou Fujian, 350002, China ; 3. College of Horticulture,Fujian Agriculture and Forestry University, Fuzhou Fujian, 350002, China);The Anti-aging Effects of Bamboo Tea Wine on D-galactose Induced Senescent Model Mice[A];[C];2013
5 ZHENG Hu-zhe 1,2 , CUI Chun-lan 1,2 (1. Center of Jiangsu Food Processing Technology, Huai’an, 223003,China;2. Jiangsu Food Science College, Huai’an, 223003, China);Effect of Flower Thinner Agent “ Eco-Huang ” to Growth and Quality on Apple Fruits[A];[C];2013
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 Shi Shuangzhi1 Li Jiang1 Wang Xingang2(1 The Food College of ShiheZi University of XinJiang,Xinjiang ShiheZi 832003;2 The ShiheZi Jinnai Food Limited company of XinJiang,Xinjiang ShiheZi 832000);The present status and prospects of walnut protein development[J];Anhui Agricultural Science Bulletin;2008-07
2 LIU Zhao-ming et al(Department of Biological and Chemical Engineering,Guangxi University of Technology,Liuzhou,Guangxi 545006);Study on Preparation and Antioxidant Activity of Hydrolysate from Neutral Protease in Walnut Protein[J];Journal of Anhui Agricultural Sciences;2008-35
3 JIANG Nan;A comparative study of carcass traits and meat characteristics of domestic rabbit[J];Journal of Chuxiong Teacher's College;2003-06
4 Tang Minmin,Jiang Zhonghang(Longdan Dairy Science and Technology Co.,Ltd,Harbin 150086);Physiological and Functional Properties of Soybean Polypeptide and Their Application in Sports Food[J];Soybean Bulletin;2006-05
5 ZHU Su-zhen,LI Fu-chang(College of Animal Science and Technology,Shandong Agricultural University,Taian 271018,China);EFFECTS OF DIGESTIBLE ENERGY LEVELS ON DIGESTIBILITY,METABOLISM AND MEAT QUALITY OF 2~3 MONTHS MEAT RABBITS[J];Acta Zoonutrimenta Sinica;2004-04
6 YI Yin 1, TAN Ai juan 2, LIU Ning 1, WANG Li ming 1(1.Department of Biology, Guizhou Normal University, Guiyang 550001; 2.Bio technical College, Guizhou University, Guiyang 550025, CHINA);Producing Technology of Papain[J];GUIZHOU AGRICULTURAL SCIENCES;2000-05
7 Deng Jing ,Wu Huachang and Zhou Jian (Biotechnology Engineering Dept., Sichuan Institute of Light Ind.&Chem.Tech.,Zigong,Sichuan 643033);Progress studies of papain[J];Guangxi Journal of Light Industry;2003-03
8 SHI Shuai,YANG Shi-zhang,NIU Lin(Animal Husbandry & Veterinary College,Taizhou 225300,China);Effect of Papain on the Tenderness of Bos grunniens Meat[J];Modern Food Science and Technology;2007-10
9 BAN Yu-feng, ZHU Hai-feng, SHUN Ming-zhu (College of Petrochemical Engineering, Shenyang University of Technology, Liaoyang 111003, China);Preparation of Soybean Oligopeptides by Alcalase-catalyzed Hydrolysis of Soybean Protein[J];Modern Food Science and Technology;2007-11
10 WANG Jing, HAO Zai-bin (College of Life Sciences,Northeast Agricultural University, Harbin 150030);Advance in Study on Properties and Function of Soybean Peptides[J];Heilongjiang Agricultural Science;2004-05
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 Gu Zilin,Chen Saijuan,Liu Yajuan,Chen Baojiang,Huang Yuting;Resources development of rabbit feed and research progress of rabbit nutrition in 2011[J];Feed Industry;2012-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Zhao Xinhuai; Fong Zhibiac(Northeast Agricultural University, Harbin 150030 China);A STUDY ON THE DETERMINATION OF THE DEGREE OF HYDROLYSIS IN SOY PROTEIN HYDROLYSATES[J];JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY;1995-02
2 YUANG Bin 1, LU Guishan 2,LIU Xiaoling 2 (1 Shenzhen Chengye Freeze Corporation Limited, Shenzhen 518019,China; 2 Biotechnology and Sugar Engineering College, Guangxi Univ.,Nanning 530004,China);The simple method of determining for the degree of hydrolysis of proteins[J];Journal of Guangxi Agricultured and Biological Science;2002-02
3 PU Shou-cheng WANG Jin-shui ( Grain College, Henan University of Technology, Zhengzhou 450052, China; College of Food and Bioengineering, China South University of Technology, Guangzhou 516450, China);Development of Preparation of Bioactive Peptides from Proteins by Enzymatic Hydrolysis[J];Journal of Cereals & Oils;2005-03
4 ;有限酶解花生蛋白的研究[J];Science and Technology of Food Industry;2004-11
5 Wu Xiao et al;Hydrolysis of extracted peanut flake protein with composite enzymes[J];Science and Technology of Food Industry;2005-01
6 Rong Jianhua et al.;Study on Antioxidtive Activities in Vitro of Soybean Peptides[J];Food Science;2002-11
7 XU Ying-cao, LIU Chun-hong (Department of Chemistry ,College of Science , Northeast Agriculture University,Harbin 150030,Heilongjiang, China);INTRODUCTION OF METHOD FOR DETERMINATION OF DEGREE OF HYDROLYSIS OF PROTEIN HYDROLYSATES[J];Food Research and Development;2007-07
8 YI Jian-hua, ZHU Zhen-bao, DONG Wen-bin (College of Bioscience and Bioengineering, Shaanxi University of Science and Technology, Xianyang 712081);Study on processing conditions of walnut protein enzymatic hydrolysis[J];Food Science and Technology;2007-03
9 ZHOU Xue -song(College of Food and Bioengineering,South China University of Technology,Guangzhou Guangdong 510640);Studies on Modification of Peanut Protein[J];;2005-03
10 WangDanxi, Zhang xinchun, Lu Xuwen, Liujinhua, Jiang Jing( 25 Hospital of PLA, Zhangj iakou 075000. P.R.China);Determination of Peptides in Tunaochuiit Injection by Biuret Colonmetry[J];China Pharmacist;2001-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved