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《Journal of Anhui Agricultural Sciences》 2009-14
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Observation of Mitosis and Meiosis in Rice Cells by Simply Prepared Glass Slide

YANG Xu et al(Henan Provincial Key Laboratory of Ion Beam Bioengineering,Zhengzhou University Zhengzhou,Henan 450052)  
[Objective] The study was to establish a set of techniques in glass slide for rice chromosome with simple procedures and to observe the cell division process of rice chromosome.[Method] Using rice root tip and anther as experimental materials,glass slides for rice chromosome were prepared by the improved method,and the processes of mitosis and meiosis in rice cell were observed under microscope and recorded by photograph.[Result] The interphase and all the four phases of mitosis including prophase,metaphase,anaphase and telophase were well observed.At metaphase,the chromosomes condensed to the minimum,thus this phase was optimal for observing and studying chromosomes.In rice cells,meiosis contained two stages of meiosis Ⅰand meiosis Π,the former included chromosome replication,whereas the latter just performed cell division.[Conclusion] The improved protocol introduced in this study could attain accurate data of rice chromosome,further providing basis for breeding and genetic manipulation.
【Fund】: 河南省自然科学基金项目(2008A208019)
【CateGory Index】: S511
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