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《Journal of Anhui Agricultural Sciences》 2009-14
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Study on the Protection Agent of Antigen to Foot and Mouth Disease Virus

LI Zheng-feng et al(Animal Medical College,Gansu Agricultural University,Lanzhou,Gansu 730070)  
[Objective] The reference for the prescription formation of the protection agents of antigen to foot and mouth disease virus(FMDV)was provided through the research on a few of the protection agents(trehalose,sucrose,sorbitol,polyvinylpyrrolidone,thiourea,gelatin,L-arginine and ascorbic acid).[Method] The best protection agent formulation of the antigen to FMDV was screened out with the duplicate pairs of orthogonal test method with three factors and three levels.The protection agent A(patent) being taken as the CK,the content of antigen-TCID50 and 146s was compared under different conditions.[Results] The content of TCID50 in the Protection Agent No.4 was significantly higher than that in the Protection Agent No.1,No.2,No.3,No.5,No.6,No.7 or No.8,respectively,with the analysis of variance.The content of TCID50 in the antigen added with the Protection Agent No.4 was 5.3,5.0,4.5 and 4.5,2.5,1.0 after the virus antigens were preserved at 4 ℃ for 90,120 and 150 days and at 37 ℃ for 40,50 and 60 hours;and added with the Protection Agent A,3.5,1.5,0.5 and 3.5、1.5,0.5,respectively.In order to further test its protective property to FMDV antigen,the content of 146s in FMDV was determined.The content of 146s in the antigens added with the Protection Agent No.4 were stored at 4 ℃ for 150 days and at 37 ℃ for 40 hours was 0.796,0.462 μg/ml,and added with the Protection Agent A,0.602,0.307 μg/ml.[Conclusion] The efficiency of the Protection Agent No.4 on FMDV antigen was better than the Protection Agent A,especially for the significant improvement of the cryo-preservative time and heat-resistant performance of FMDV.
【Fund】: 国家支撑计划项目(206BAD06A06)
【CateGory Index】: S852.5
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