Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui Agricultural Sciences》 2009-15
Add to Favorite Get Latest Update

Experiment Study on the Browning Prevention Technology in Tissue Culture of Iris germanica

WANG Wen-jing et al(Zhengzhou College of Animal Husbandry Engineering,Zhengzhou,Henan 450011)  
[Objective] The research aimed to study the browning prevention techniques in tissue culture of Iris germanica.[Method] With three varieties of Sun Dou,Bloodstone and Immortality of German I.germanica as cultured material,and MS+15 g/L sucrose + 8 g/L agar powder as basic medium,the effect of browning factor and browning prevention techniques in tissue culture of I.germanica was studied.[Result] The new buds and petals of I.germanica was proper to be used as explant of tissue culture,with lower browning rate compared with other positions.The well situated tissue block of explant(3 mm×3 mm×3 mm) might reduce browning rate.Adding 0.1%Vc or 0.3% active carbon in medium had obvious effect on preventing tissue browning.The exogenous hormone 2 mg/L 6-BA was easy to lead to brownness turning,and 2 mg/L Kt and 2 mg/L NAA brought less effect to tissue browning.When using disinfectants of alcohol,its dipping time of 5~10 s for explant was the best,with the minimum browning rate.The culture under full illumination condition was liable to induce brownness turning,compared with other cultured conditions,the brownness turning could be restrained effectively by using imitating artificial climate condition for culture.[Conclusion] The study provided reference for research of industrial seedling of I.germanica.
【Fund】: 河南省科技攻关项目(072102110010)
【CateGory Index】: S682.19
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved