Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Anhui Agricultural Sciences》 2009-15
Add to Favorite Get Latest Update

A Simple and Effective Chemical Modification Method for Extracting DNA from Fungi

GU Yan-chang et al(College of Life Sciences,Henan Normal University,Xinxiang,Henan 453007)  
[Objective] The aim was to explore the effective method suitable for the extraction of fungal genomic DNA.[Method] With Hansenula sp.HS07A and Penicillium sp.HS07B as tested strains,boiling lysis method,quartz sand grinding method,grinding and repeated grinding and freeze-thaw method,and chemical modification method were adopted for the extraction of their genomic DNA and the extraction effect of the 4 methods were compared.[Result] Using chemical modification method extracting genomic DNA could eliminate the process of machine wall-breaking,reduce the damage of mechanical force on DNA.Using anionic detergent SDS for DNA extraction was safe and cheap,which could extract high quality genomic DNA effectively and completely.The gel electrophoretic band was clear and had no dispersion,and using the genomic DNA as the template for PCR amplification of target segment,the amplification effect was better than that by the method before modification and the method was still suitable for the genomic DNA extraction of many kinds of epiphyte and bacteria.[Conclusion] Chemical modification method was an effective method for fungal genomic DNA extraction.
【Fund】: 河南省教育厅科技攻关项目(2007610004);; 河南省科技厅科技攻关项目(082102220014)
【CateGory Index】: S188
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 2 Hits
1 Yan Li~1,Sun Xiu-lan~1,Zhang Yin-zhi~2,Zhao Jian-xin~1,Wang Miao~1 1(School of Food Science & Technology,Jiangnan University,Wuxi 214036,China) 2(The State Key Lab of Food Science and Technology,Jiangnan University,Wuxi 214122,China);Research of Quantification of the Copy Number of nor-1,a Gene of the Aflatoxin Biosynthetic Pathway,in Fermented Bean Sauce by Real-time PCR[J];Food and Fermentation Industries;2011-11
2 AN Xiao-qing*,ZHOU Yong-an,SU Li-Ping,et al.*Shanxi Medical University,Taiyuan 030001,China;Indetification fungal in clinical blood samples By combinging PCR and RFLP[J];National Medical Frontiers of China;2011-03
【Citations】
Chinese Journal Full-text Database 4 Hits
1 YANG Yue-mei,JIANG Xian-zhang,HUANG Jian-zhong(College of Life Sciences,Fujian Normal University,Fuzhou 350108,China);Comparison of Different Methods for the Isolation of Genomic DNA of Penicillium expansum PF898[J];Journal of Fujian Normal University(Natural Science Edition);2007-02
2 YANG Lian-ru~1, YANG Xiao-ye~1, LIU Zhen-lian~1, KAO Gui-lan~1, LI Qiao-e~1,WANG Jun~2 (1.College of Animal Science and Medicine,Inner Mongolia Agricultural University,Huhhot 010018,China; 2.Inner Mongolia Agricultural school,Huhhot 010070,China);THE EXPERIMENT OF DNA EXTRACTION AND 18S rDNA SEQUENCE AMPLIFICATION OF Arthrobotrys oligosproa[J];Journal of Inner Mongolia Institute of Agriculture and Animal Husbandry;2004-03
3 CUI Lixia,HAN Jianrong (School of Life Science and Technology, Shanxi University,Taiyuan 030006,China);Comparision of Methods of Extracting DNA from Penicillium[J];Journal of Shanxi University (Natural Science Edition);2004-02
4 He Ye-xuan~1,Wang Shi-ying~2,Li Bing~3 and Zhu Bao-cheng~(1,2 *) (1 College of Life Science,Hebei University,Baoding 071002;2 College of Life Science,The Agricultural University of Hebei,Baoding 071001;3 Xingtai Medical College,Xingtai 054056);A new method for rapid extraction of chromosomal DNA from Penicillium chrysogenum[J];Chinese Journal of Antibiotics;2005-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LI Yu-hui et al(Department of Biological and Environmental Engineering,Hefei University,Hefei,Anhui 230022);Screening and Identification of a Nitrogen-phosphorus Degradation Strain[J];Journal of Anhui Agricultural Sciences;2009-16
2 CHEN Ben-liang,ZHANG Qi-zhong(Institute of Hydrobiology,Jinan University,Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering,Ministry of Education,Key Laboratory of Aquatic Eutrophication and Control of Harmful Algal Blooms of Guangdong Higher Education Institutes,Guangzhou 510632,China);Progress on Research of Saprolegnia and Saprolegniasis Treatment in Fish[J];Fisheries Science;2011-07
3 YUAN Hong-shui1,LI Shu-na1,ZHANG Ai-lian2,ZHOU Zhi-jun1,WANG Shi-ying1,ZHU Bao-cheng1,2(1.College of Life Science,Agricultural University of Hebei,Baoding 071001,China;2.College of Life Science,Hebei University,Baoding 071002,China);Quartzite sand grind mycelia for rapid preparation of chromosome DNA of Cephalosporium acremonium[J];Journal of Agricultural University of Hebei;2007-05
4 LI Jing,KE Chong-rong,YANG Xin-wei,TIAN Bao-yu,HUANG Jian-zhong*(Engineering Research Center of Industrial Microbiology,Ministry of Education,College of Life Sciences,Fujian Normal University,Fuzhou 350108,China);Comparative Analysis of 18S rRNA Gene Sequences between the High Griseofulvin Producing Mutant and Its Original Strain[J];Journal of Fujian Normal University(Natural Science Edition);2008-03
5 YANG Xin-wei,LIN Wei-lin,TIAN Bao-yu,KE Chong-rong,HUANG Wei,HUANG Jian-zhong*(Engineering Research Center of Industrial Microbiology,Ministry of Education,Fujian Normal University,Engineering Research Center of Fujian Modern Fermentation Technology,Fuzhou 350108,China);Identification of Aspergillus niger EIM-6 with High-yield of Pectinase and Optimization of Its Submerged Fermentation Conditions by Response Surface Methodology[J];Journal of Fujian Normal University(Natural Science Edition);2009-02
6 WEI Fang,LIN Yan-huan,YE Bing-ying,ZHANG Ji-sen,CHEN You-qiang(College of Life Sciences,Fujian Normal University/ Key Laboratory of Sugarcane Genetic Improvement,Ministry of Agriculture,Fuzhou,Fujian 350108,China);Screening and identifying of xylanase producing strain from sugarcane rhizosphere soil[J];Subtropical Agriculture Research;2011-03
7 HUANG Yang et al(Engineering Research Center of Industrial Microbiology,Ministry of Education College of Life Sciences,Engineering Research Center of Fujian Modern Fermentation Technology,Fujian Normal University,Fuzhou,Fujian 350108);Medium Optimization for Liquid Fermentation of Hirsutella sinensis[J];Journal of Anhui Agricultural Sciences;2013-01
8 NIU Li-na,WANG Ying,RAO Lang-yu,PEI Hua,PENG Jiang-long,CHEN Jin-long,LV Gang,LIN Ying-zi (School of Tropical and Laboratory Medicine Hainan Medical University,Haikou Hainan 571101,China);Classification and identification of mangrove fungi strain WC1016 possessing immunity activity[J];Journal of Hainan Medical University;2011-08
9 SUN Li-Fu1, 2 ZHANG Yan-Hua1 PEI Ke-Quan2 1Life Science Faculty, Shaoxing College of Arts & Sciences, Shaoxing 312000, China 2 Key Laboratory of Vegetation and Environmental Change, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China;A rapid extraction of genomic DNA from fungi[J];Mycosystema;2009-02
10 WU Han-Qin SU Jing-Qian XIE Ming-Ying YANG Min-He College of Life Sciences, Fujian Normal University, Fuzhou 350108, China;An endophytic Trichoderma species from Camellia sinensis:its characterization and endophytism[J];Mycosystema;2009-03
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 CHEN Jian-shan et al (Environment and Plant Protection College,SCUTA,Danzhou,Hainan 571737);Application of ITS Sequences in Fungi Classification and Identification[J];Journal of Anhui Agricultural Sciences;2007-13
2 SHEN Yi et al(College of Plant Protection,Anhui Agricultural University,Hefei,Anhui 230036);Preparation and Purification of Trichodema Chitinase and Chitooligosaccharide[J];Journal of Anhui Agricultural Sciences;2009-16
3 XU Zhong-bo,REN Xiao-ming(Department of Animal Science and Veterinary Medicine,Beijing University of Agriculture,Beijing 102206,China);Review on Biological Properpty and Selection Standard of Probiotics Strain[J];Journal of Beijing University of Agriculture;2007-04
4 MAO Zhi qun,ZHANG Wei,MA Wen (College of Food Science and Technology, Agricultural University of Hebei, Baoding 07100, China);The utilization of molecular biology in yeast taxonomy[J];Journal of Agricultural University of Hebei;2002-S1
5 WANG Yang, LOU Yong-jiang, YANG Wen-ge (Faculty of Food Science and Engineering, Ningbo University,Ningbo 315211, China);Prespect of studying in enzymatic hydrolysising chitin and chitosan[J];Donghai Marine Science;2001-04
6 MA Zhi-ke,ZAN Lin-sen(College of Animal Science Technologe,Northwest A & F University,Yangling,Shaanxi,712100,China);Progress on Hazard,Detection Techniques and Biodegradation of Aflatoxin[J];Progress in Veterinary Medicine;2009-09
7 ZHANG Wen~1, NI Li~1, CHEN Li-yun~2, CAI Wei~1, ZHANG Shao-sheng~2 (1.Institute of Biotechnology, Fuzhou University, Fuzhou, Fujian 350002, China; 2.Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, F ujian Agriculture and Forestry Un iversity, Fuzhou, Fujian 350002, China);Optimizing conditions of chitinase production from Paecilomyces lilacinus strain FZ-0289[J];;2005-02
8 Yang Yuhuan; Yan Shihuang; Lu Deqing(Guizhou Acadeny of Agricultural Sciences Guiyang 550006);STUDY ON CONTROL OF SOUTHERN STEM ROT AND DAMPING OFF BY TRICHODERMAIN HOT PEPPER[J];GUIZHOU AGRICULTURAL SCIENCES;1996-02
9 Jiao Dezhi Zhu Yunlan(Keshan Junior Teachers College);Improvment of Method of Preparation of Microsection about Yeast Ascospore[J];JOURNAL OF SCIENCE OF TEACHERS COLLEGE AND UNIVERSITY;2000-03
10 CHEN Fang-chun1, LIN Mei2. (1. Dept. of Oral Medicine, The Affiliated Hospital of Stomatology, Chongqing University of Medical Sciences, Chongqing 400015, China; 2. Dept. of Oral Medicine, West China College of Stomatology, Sichuan University, Chengdu 610041, China);Candida species changing and drug resistance of Candida strains to antifungal agents[J];International Journal of Stomatology;2007-05
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 LIU Feng-liang1,ZHAO Zhi-hui2,XIE Jing1(1.College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China; 2.Institute for Agri-food Standards and Testing Technology,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China);Research progress of mycotoxins in cereals[J];Guangdong Agricultural Sciences;2012-19
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 ZHANG Li li,ZHANG Ling hua,SHI Jian fei,WANG Yun ji (Dept. of Food Sci. and Biotechnol., Dalian Inst. of Light Ind., Dalian 116001,China);Isolation and molecular biological analyses of genomic DNAs from fungi using benzyl chloride[J];JOURNAL OF DALIAN INSTITUTE OF LIGHT INOUSTRY;2000-01
2 TANG GUO-MIN (Institute of Microbiology, Academia Sinica, Beijing 100080);ADVANCES IN GENE EXPRESSION SYSTEM OF FILAMENTOUS FUNGI[J];Mycosystema;1992-02
3 Zhu Heng Qu Feng Zhu Lihuang (Institute of Genetics, Academta Sinica, Beijing 100101);ISOLATION OF GENOMIC DNAS FROM FUNGI USING BENZYL CHLORIDE[J];Mycosystema;1994-01
4 LIN Lin 1),2),3)* , XIE Bi feng 3) , YANG Guan zhen 2) , SHI Qiao qin 3) , LIN Qi ying 1) , XIE Lian hui 1) , WU Song gang 3) , WU Xiang fu 2) * ( 1) Institute of Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2) Shanghai Institute of Biochemistry, Chinese Academy of Sciences,Shanghai 200031, China; 3) Bioengineering College, Fujian Normal University, Fuzhou 350007, China);Cloning and Sequencing of Genomic DNA Encoding Alkaline Lipase from Penicillium expansum PF898[J];Chinese Journal of Biochemistry and Molecular Biology;2003-01
5 Han Ligang (Department of Nosetiology,Nangjing Military Medical College,Nangjing,210099) Yuan Yi Wang Gang (Department of Agronomy,University of Agriculture and Animal Science of PLA,Changchun,130062);EXTRACTION OF DNA FROM FILAMENTOUS FUNGAL TISSUES[J];BIOTECHNOLOGY;1999-06
6 SHAN Zhi-ping,MENG Yu,JIANG Wen-hou (Jiangsu Institute of Microbiology,Wuxi 214063);Research on distilling DNA from silk shape fungus Blakeslea trispora[J];Biotechnology;2001-03
7 CUI Lixia,HAN Jianrong (School of Life Science and Technology, Shanxi University,Taiyuan 030006,China);Comparision of Methods of Extracting DNA from Penicillium[J];Journal of Shanxi University (Natural Science Edition);2004-02
8 Zhu Baocheng;Cheng Yali;Li Qingyu(Hebei University,Baoding 071002)Wang Jungang;Zhao Suying;Shi Jing;Li Liangsheng(Hebei Pharmaceutical Factory,Shijiazhuang 050041);SCREENING OF HIGH-POTENCY PENICILLIN PRODUCING STRAIN BY UV IRRADIATION OF PROTOPLASTS FROM PENICILLUM CHRYSOENUM[J];CHINESE JOURNAL OF NATIBIOTICS;1994-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved