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《Journal of Anhui Agricultural Sciences》 2009-15
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A Simple and Effective Chemical Modification Method for Extracting DNA from Fungi

GU Yan-chang et al(College of Life Sciences,Henan Normal University,Xinxiang,Henan 453007)  
[Objective] The aim was to explore the effective method suitable for the extraction of fungal genomic DNA.[Method] With Hansenula sp.HS07A and Penicillium sp.HS07B as tested strains,boiling lysis method,quartz sand grinding method,grinding and repeated grinding and freeze-thaw method,and chemical modification method were adopted for the extraction of their genomic DNA and the extraction effect of the 4 methods were compared.[Result] Using chemical modification method extracting genomic DNA could eliminate the process of machine wall-breaking,reduce the damage of mechanical force on DNA.Using anionic detergent SDS for DNA extraction was safe and cheap,which could extract high quality genomic DNA effectively and completely.The gel electrophoretic band was clear and had no dispersion,and using the genomic DNA as the template for PCR amplification of target segment,the amplification effect was better than that by the method before modification and the method was still suitable for the genomic DNA extraction of many kinds of epiphyte and bacteria.[Conclusion] Chemical modification method was an effective method for fungal genomic DNA extraction.
【Fund】: 河南省教育厅科技攻关项目(2007610004);; 河南省科技厅科技攻关项目(082102220014)
【CateGory Index】: S188
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