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《Journal of Anhui Agricultural Sciences》 2009-16
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Investigation and Analysis of the Endophyte Bacteria of Wild Orchids in Hainan Province

WANG Feng et al(Hainan University,Danzhou,Hainan 571737)  
[Objective] The antagonistic activity of endophyte bacteria was hoped to be isolated through their isolation and identification from some wild orchids varieties in Henan.[Method] The endophyte bacteria were isolated with the tissue culture method and the biological character of them was identified with the method of 16 S rDNA.[Result] The isolators from 7 bacterial strains had the antagonistic activity,among which,the prominent antagonistic activity appeared in 2 strains,named as WF1 and WF2.The WF1 and WF2 were selected under the antibiotic pressure with the different concentrations of rifampicin and the WF1 was the strain with the tolerance to antibiotic,which was used in the experiment in inoculation.[Conclusion] After the total DNA was extracted,its recovery,connection and transformation were done.The sequence of each 16 S rDNA gene at the 1 418 bp could be found in the GenBank through the test of positive clone from the end of the primer of CW34668 and the similar reached to 99.5%,which make sure that the strain belonged to Bacillus.
【Fund】: 海南大学作物遗传重点学科资助项目
【CateGory Index】: S182
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