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《Journal of Anhui Agricultural Sciences》 2009-16
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Studies on the Extraction of Polysaccharides from Sargassum and Its Immunological Experiment

ZHOU Zhen-bing et al (Guangxi Agricultural Vocational and Technical College,Nanning,Guangxi 530007)  
[Objective]The research aimed to study the extraction process of polysaccharides from sargassum and its immunological experiment.[Method] With the spectific sargassum species in Weizhoudao,Beihai in Guangxi as experimental materials,the optimum technical conditions of extracting polysaccharides from sargassum were explored,and the immunological experiment of sargassum polysaccharides was tested.[Result]The results indicated that the optimum technical conditions of extracting polysaccharides from sargassum as follows: water-bath temperature 80 ℃,extraction time 10 h,the time of treating sargassum with colloid grinding was 2 min,the suitable proportion of algae powder and water was 1∶20 and the ration of the concentrated liquid volume number and 95% alcohol was 1.0∶3.0.Immunological experiments showed that sargassum polysaccharides could increase the weight of the spleen of mice significantly(P0.05) when the dose of polysaccharides reached 200,400 and 600 mg/kg·d.It could significantly increased the weight of the lymphonodi axillares,strengthened the phagocytosis function of monocytle-macrophages,raised the clearance rate of mice charcoal particles,and promote the index of phagocytic(a) significantly(P0.05),and had significant effects on the macrophages hyperplasia and the shape of lymph nodule when the dose of polysaccharides was 400,600 mg/kg·d.The significant lymph nodule in lymph node was founded,but also a large number of macrophages were formed when the dose of polysaccharides was 600 mg/kg·d.[Conclusion] This research will provide the referenced basis for the further study of sargassum physiological activity materials-polysaccharides.
【Fund】: 海洋生物技术应用研究子课题:广西沿海海藻药用成分提取及其生物活性的研究(桂科攻0428005-19A)
【CateGory Index】: R284
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