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《Journal of Anhui Agricultural Sciences》 2009-17
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Isolation and Screening of Strains Producing Cellulase and Their Enzyme Activity Determination

WU Lin et al (Institute of Environment and Plant Protection,Chinese Academy of Tropical Agricultural Science,Danzhou,Hainan 571737)  
[Objective] The purpose was to find more new high-efficiency cellulose-decomposing strains and their utilization methods.[Method] The strains with stronger cellulose-decomposing ability were screened out by the method "sampling — culture — isolation of single colony — primary screening — secondary screening — determination of OD value".[Result] Thirty-five strains with cellulose-decomposing ability were isolated from 80 samples through repeated culture and streaking in primary screening.Among them,10 strains turned from white to be green and their growth potentials were better;6 strains were dark green and their growth potentials were common;4 strains were green and their growth potentials were better;all these 20 strains produced spores and were identified to be Trichoderma viride preliminarily.The congo-red media were used and 9 strains with bigger transparent circles were got in secondary screening.These 9 strains were cultured by fermentation and their enzyme activities were determined by DNS method,then 2 strains with stronger enzyme activity were got.These 2 strains were identified to be Trichoderma asperellum.Twenty strains with stronger cellulose-decomposing ability were screened out through filter-paper disintegration test.The results of enzyme activity determination by DNS method showed that the inulinase-producing activities of the 2 strains collected from the stacking place of sugar cane and sugar cane field were highest.[Conclusion] The 2 strains collected from the stacking place of sugar cane and sugar cane field could be used as new cellulose-decomposing strains.
【Fund】: 国家科技支撑计划项目(2007BAD48B02)
【CateGory Index】: TQ920.1
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