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《Journal of Anhui Agricultural Sciences》 2009-19
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Research on the Poplar Transferred of AtNHX1 Gene with the cre/lox Expression Vector

YANG Yang et al(College of Life Sciences,Nanjing Xiaozhuang University,Nanjing,Jiangsu 211171)  
[Objective] The poplar transferred with Na+/H+ anti-porter AtNHX1 gene was studied.[Method] The leaf of the poplar variety——Nanlin 895 being taken as explants,the expression vector cre/lox was set up with Agrobacterium-mediated method,and 3 transgenic plants were detected at molecular level with PCR.[Results] The results showed that the condition of suitable conversion was as follows that after the explants were pre-cultured in the differentiation medium for 2 days,its soaking time in the bacterium with OD600 nm value of 0.3-0.4 was 7 minutes.And then,the ratio of kanamycin-resistant green plant could be 43.9%.PCR molecular detection showed that the target gene had been integrated into poplar genome.[Conclusion] The system could be applied in gene transformation of poplar
【Fund】: 南京晓庄学院重点项目(2007NXY01);; 生态学校级重点学科项目(2005-2008);; 江苏省高校自然科学基金项目(08KDJ180011);; 江苏省高等学校大学生实践创新训练计划项目(2007-2009)
【CateGory Index】: S792.11
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