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《Journal of Anhui Agricultural Sciences》 2009-19
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Study on Inheritance of HMW-GS in F_2 Grain of Jinmai

ZHANG Li et al(College of Arts and Science,Shanxi Agricultural University,Taigu,Shanxi 030801)  
[Objective] The aim was to provide the basis for forecasting processing quality of hybrid wheat and combining parents in high quality wheat breeding process.[Method] With grains of female parent Jinmai 47,male parent Fengyou 1 and F2 of hybrid wheat as the materials,the isolation and expression of high molecular weight glutenin subunits(HMW-GS) in grains were analyzed by SDS-PAGE electrophoresis.[Result] 21 kinds of band phenotypes of HMW-GS appeared in F2 grains.Among which band phenotypes that regressed to female parent(N,7+9,3+12) was 69.10%,and that regressed to male parent(1、17+18、5+10) was 1.04%,the remained 29.86% band phenotypes were hybrid band phenotypes and deletion band phenotypes..HMW-GS isolated in F2 grains according to a certain inheritance,Glu-Dlb and Glu-Dld was recombined(recombination rate of 5.90%),17+18 and 7+9 subunits expressed at Glu-B1 site at the same time,while there were no expression of Glu-Blc at this site(vacancy rate of 5.20%).[Conclusion] This study determined the law of expression and isolation of HMW-GS in F2 grain of wheat.
【Fund】: 山西农业大学青年基金项目(2008012)
【CateGory Index】: S512.1
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